RRC ID 4007
著者 Lee MH, Kim B, Song SK, Heo JO, Yu NI, Lee SA, Kim M, Kim DG, Sohn SO, Lim CE, Chang KS, Lee MM, Lim J.
タイトル Large-scale analysis of the GRAS gene family in Arabidopsis thaliana.
ジャーナル Plant Mol Biol
Abstract GRAS proteins belong to a plant-specific transcription factor family. Currently, 33 GRAS members including a putative expressed pseudogene have been identified in the Arabidopsis genome. With a reverse genetic approach, we have constructed a "phenome-ready unimutant collection" of the GRAS genes in Arabidopsis thaliana. Of this collection, we focused on loss-of-function mutations in 23 novel GRAS members. Under standard conditions, homozygous mutants have no obvious morphological phenotypes compared with those of wild-type plants. Expression analysis of GRAS genes using quantitative real-time RT-PCR (qRT-PCR), microarray data mining, and promoter::GUS reporter fusions revealed their tissue-specific expression patterns. Our analysis of protein-protein interaction and subcellular localization of individual GRAS members indicated their roles as transcription regulators. In our yeast two-hybrid (Y2H) assay, we confirmed the protein-protein interaction between SHORT-ROOT (SHR) and SCARECROW (SCR). Furthermore, we identified a new SHR-interacting protein, SCARECROW-LIKE23 (SCL23), which is the most closely related to SCR. Our large-scale analysis provides a comprehensive evaluation on the Arabidopsis GRAS members, and also our phenome-ready unimutant collection will be a useful resource to better understand individual GRAS proteins that play diverse roles in plant growth and development.
巻・号 67(6)
ページ 659-70
公開日 2008-8-1
DOI 10.1007/s11103-008-9345-1
PMID 18500650
MeSH Arabidopsis / genetics* Arabidopsis / growth & development Arabidopsis / metabolism Arabidopsis Proteins / classification Arabidopsis Proteins / genetics* Arabidopsis Proteins / physiology* Cell Nucleus / chemistry Gene Expression Multigene Family Mutation Plants, Genetically Modified / metabolism RNA, Messenger / metabolism Transcription Factors / classification Transcription Factors / genetics* Transcription Factors / physiology* Two-Hybrid System Techniques
IF 3.302
引用数 101
WOS 分野 PLANT SCIENCES BIOCHEMISTRY & MOLECULAR BIOLOGY
リソース情報
シロイヌナズナ / 植物培養細胞・遺伝子 pst16033 pst13527 pst12157 pst15116 pst14184 pst10499 pst10702