RRC ID |
4007
|
著者 |
Lee MH, Kim B, Song SK, Heo JO, Yu NI, Lee SA, Kim M, Kim DG, Sohn SO, Lim CE, Chang KS, Lee MM, Lim J.
|
タイトル |
Large-scale analysis of the GRAS gene family in Arabidopsis thaliana.
|
ジャーナル |
Plant Mol Biol
|
Abstract |
GRAS proteins belong to a plant-specific transcription factor family. Currently, 33 GRAS members including a putative expressed pseudogene have been identified in the Arabidopsis genome. With a reverse genetic approach, we have constructed a "phenome-ready unimutant collection" of the GRAS genes in Arabidopsis thaliana. Of this collection, we focused on loss-of-function mutations in 23 novel GRAS members. Under standard conditions, homozygous mutants have no obvious morphological phenotypes compared with those of wild-type plants. Expression analysis of GRAS genes using quantitative real-time RT-PCR (qRT-PCR), microarray data mining, and promoter::GUS reporter fusions revealed their tissue-specific expression patterns. Our analysis of protein-protein interaction and subcellular localization of individual GRAS members indicated their roles as transcription regulators. In our yeast two-hybrid (Y2H) assay, we confirmed the protein-protein interaction between SHORT-ROOT (SHR) and SCARECROW (SCR). Furthermore, we identified a new SHR-interacting protein, SCARECROW-LIKE23 (SCL23), which is the most closely related to SCR. Our large-scale analysis provides a comprehensive evaluation on the Arabidopsis GRAS members, and also our phenome-ready unimutant collection will be a useful resource to better understand individual GRAS proteins that play diverse roles in plant growth and development.
|
巻・号 |
67(6)
|
ページ |
659-70
|
公開日 |
2008-8-1
|
DOI |
10.1007/s11103-008-9345-1
|
PMID |
18500650
|
MeSH |
Arabidopsis / genetics*
Arabidopsis / growth & development
Arabidopsis / metabolism
Arabidopsis Proteins / classification
Arabidopsis Proteins / genetics*
Arabidopsis Proteins / physiology*
Cell Nucleus / chemistry
Gene Expression
Multigene Family
Mutation
Plants, Genetically Modified / metabolism
RNA, Messenger / metabolism
Transcription Factors / classification
Transcription Factors / genetics*
Transcription Factors / physiology*
Two-Hybrid System Techniques
|
IF |
3.302
|
引用数 |
101
|
WOS 分野
|
PLANT SCIENCES
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
リソース情報 |
シロイヌナズナ / 植物培養細胞・遺伝子 |
pst16033
pst13527
pst12157
pst15116
pst14184
pst10499
pst10702 |