RRC ID |
51985
|
著者 |
Kamei R, Fujimura T, Matsuda M, Kakihara K, Hirakawa N, Baba K, Ono K, Arakawa K, Kawamoto S.
|
タイトル |
A flavanone derivative from the Asian medicinal herb (Perilla frutescens) potently suppresses IgE-mediated immediate hypersensitivity reactions.
|
ジャーナル |
Biochem Biophys Res Commun
|
Abstract |
Perilla frutescens is a dietary leafy herb consumed as a traditional Japanese condiment as well as used for Chinese medicine with anti-inflammatory activity. Here we report a hitherto-unrecognized P. frutescens phytochemical that potently suppresses IgE-mediated type I hypersensitivity reactions. Structural analysis reveals that the purified anti-allergic compound (Perilla-derived methoxyflavanone, PDMF) is identified as 8-hydroxy-5,7-dimethoxyflavanone. PDMF significantly inhibits IgE-mediated histamine release from RBL-2H3 rat basophilic leukemia cells as compared with those seen in known P. frutescens-derived anti-inflammatory polyphenols. We also show that oral administration of PDMF not only suppresses passive cutaneous anaphylaxis, but also prevents allergic rhinitis-like nasal symptoms in a murine model of Japanese cedar pollinosis. Mechanistically, PDMF negatively regulates Akt phosphorylation and intracellular Ca2+ influx, both of which are essential for mast cell secretory granule translocation and its exocytosis upon high-affinity IgE receptor (FcεRI) cross-linking. These results represent PDMF as a new potent anti-allergic phytochemical useful for prevention of IgE-driven hypersensitivity reactions.
|
巻・号 |
483(1)
|
ページ |
674-679
|
公開日 |
2017-1-29
|
DOI |
10.1016/j.bbrc.2016.12.083
|
PII |
S0006-291X(16)32127-1
|
PMID |
27986566
|
MeSH |
Animals
Anti-Allergic Agents / administration & dosage*
Anti-Allergic Agents / chemistry
Anti-Allergic Agents / isolation & purification
Anti-Allergic Agents / pharmacology
Cell Line, Tumor
Cryptomeria / immunology
Disease Models, Animal
Drugs, Chinese Herbal / administration & dosage*
Drugs, Chinese Herbal / chemistry
Drugs, Chinese Herbal / isolation & purification
Drugs, Chinese Herbal / pharmacology
Female
Flavanones / administration & dosage*
Flavanones / chemistry
Flavanones / isolation & purification
Flavanones / pharmacology
Histamine Release / drug effects
Hypersensitivity, Immediate / prevention & control*
Immunoglobulin E / immunology*
Mice
Mice, Inbred BALB C
Passive Cutaneous Anaphylaxis / drug effects
Perilla frutescens / chemistry*
Plants, Medicinal / chemistry*
Rats
Receptors, IgE / immunology
Rhinitis, Allergic, Seasonal / drug therapy*
Rhinitis, Allergic, Seasonal / prevention & control
|
IF |
2.985
|
引用数 |
9
|
リソース情報 |
ヒト・動物細胞 |
RBL 2H3(RCB2782) |