RRC ID 51985
著者 Kamei R, Fujimura T, Matsuda M, Kakihara K, Hirakawa N, Baba K, Ono K, Arakawa K, Kawamoto S.
タイトル A flavanone derivative from the Asian medicinal herb (Perilla frutescens) potently suppresses IgE-mediated immediate hypersensitivity reactions.
ジャーナル Biochem Biophys Res Commun
Abstract Perilla frutescens is a dietary leafy herb consumed as a traditional Japanese condiment as well as used for Chinese medicine with anti-inflammatory activity. Here we report a hitherto-unrecognized P. frutescens phytochemical that potently suppresses IgE-mediated type I hypersensitivity reactions. Structural analysis reveals that the purified anti-allergic compound (Perilla-derived methoxyflavanone, PDMF) is identified as 8-hydroxy-5,7-dimethoxyflavanone. PDMF significantly inhibits IgE-mediated histamine release from RBL-2H3 rat basophilic leukemia cells as compared with those seen in known P. frutescens-derived anti-inflammatory polyphenols. We also show that oral administration of PDMF not only suppresses passive cutaneous anaphylaxis, but also prevents allergic rhinitis-like nasal symptoms in a murine model of Japanese cedar pollinosis. Mechanistically, PDMF negatively regulates Akt phosphorylation and intracellular Ca2+ influx, both of which are essential for mast cell secretory granule translocation and its exocytosis upon high-affinity IgE receptor (FcεRI) cross-linking. These results represent PDMF as a new potent anti-allergic phytochemical useful for prevention of IgE-driven hypersensitivity reactions.
巻・号 483(1)
ページ 674-679
公開日 2017-1-29
DOI 10.1016/j.bbrc.2016.12.083
PII S0006-291X(16)32127-1
PMID 27986566
MeSH Animals Anti-Allergic Agents / administration & dosage* Anti-Allergic Agents / chemistry Anti-Allergic Agents / isolation & purification Anti-Allergic Agents / pharmacology Cell Line, Tumor Cryptomeria / immunology Disease Models, Animal Drugs, Chinese Herbal / administration & dosage* Drugs, Chinese Herbal / chemistry Drugs, Chinese Herbal / isolation & purification Drugs, Chinese Herbal / pharmacology Female Flavanones / administration & dosage* Flavanones / chemistry Flavanones / isolation & purification Flavanones / pharmacology Histamine Release / drug effects Hypersensitivity, Immediate / prevention & control* Immunoglobulin E / immunology* Mice Mice, Inbred BALB C Passive Cutaneous Anaphylaxis / drug effects Perilla frutescens / chemistry* Plants, Medicinal / chemistry* Rats Receptors, IgE / immunology Rhinitis, Allergic, Seasonal / drug therapy* Rhinitis, Allergic, Seasonal / prevention & control
IF 2.985
引用数 9
リソース情報
ヒト・動物細胞 RBL 2H3(RCB2782)