RRC ID 52734
著者 Okuzaki Y, Kaneoka H, Nishijima KI, Murakami S, Ozawa Y, Iijima S.
タイトル Molecular cloning of chicken TET family genes and role of chicken TET1 in erythropoiesis.
ジャーナル Biochem Biophys Res Commun
Abstract Ten-eleven translocation (TET) methylcytosine dioxygenase has potential as an active eraser to regulate the genomic DNA methylation status. We herein cloned chicken TET (cTET) family genes, and confirmed their functions. Quantitative reverse-transcription PCR showed that cTET1 was strongly expressed in erythrocytes throughout development. This cTET1 expression pattern, together with the results of methylated or hydroxymethylated DNA immunoprecipitation, suggests that cTET1 contributes to demethylation around the promoter region of the definitive-type β-globin gene βΑ in erythroid cells. The knockdown of cTET1 in T2ECs chicken erythroid progenitor cells suppressed the induction of βΑ expression under differentiation conditions. These results suggest that cTET1 plays an important role in erythroid cell differentiation.
巻・号 490(3)
ページ 753-759
公開日 2017-8-26
DOI 10.1016/j.bbrc.2017.06.113
PII S0006-291X(17)31244-5
解説 ニワトリのTET1をクロニーニングし、その機能を調べた。その結果赤血球の分化に重要な役割を持っていることが示唆された。
PMID 28642138
MeSH 5-Methylcytosine / analogs & derivatives 5-Methylcytosine / metabolism* Animals Cell Line Chickens / genetics* Chickens / physiology Cloning, Molecular* DNA Methylation Dioxygenases / genetics* Dioxygenases / metabolism Erythropoiesis* HeLa Cells Humans Multigene Family Promoter Regions, Genetic beta-Globins / genetics
IF 2.985
引用数 0
リソース情報
ニワトリ・ウズラ