RRC ID 50677
著者 Okanishi H, Kim K, Fukui K, Yano T, Kuramitsu S, Masui R.
タイトル Proteome-wide identification of lysine succinylation in thermophilic and mesophilic bacteria.
ジャーナル Biochim Biophys Acta Proteins Proteom
Abstract Lysine succinylation, one of post-translational acylations conserved from eukaryotes to bacteria, plays regulatory roles in various cellular processes. However, much remains unknown about the general and specific characteristics of lysine succinylation among bacteria, and about its functions different from those of other acylations. In this study, we characterized lysine succinylation, a newly discovered widespread type of lysine acylation in five bacterial species with different characteristics such as optimal growth temperature and cell wall structure. This study is the first to demonstrate that succinylation is general phenomenon occurring not only in mesophiles but also in thermophiles. Mapping of succinylation sites on protein structures revealed that succinylation occurs at many lysine residues important for protein function. Comparison of the succinylation sites in the five bacterial species provides insights regarding common protein regulation mechanisms utilizing lysine succinylation. Many succinylation sites were conserved among five bacteria, especially between Geobacillus kaustophilus and Bacillus subtilis, some of which are functionally important sites. Furthermore, systematic comparison of the succinyl-proteome results and our previous propionyl-proteome results showed that the abundance of these two types of acylations is considerably different among the five bacteria investigated. Many succinylation and propionylation events were detected in G. kaustophilus, whereas Escherichia coli and B. subtilis exhibited high succinylation and low propionylation; low succinylation and high propionylation were identified in Thermus thermophilus, and low succinylation and propionylation were observed in Rhodothermus marinus. Comparison of the characteristics of lysine succinylation and lysine propionylation suggested these two types of acylation play different roles in cellular processes.
巻・号 1865(2)
ページ 232-242
公開日 2017-2-1
DOI 10.1016/j.bbapap.2016.11.009
PII S1570-9639(16)30246-1
PMID 27888076
MeSH Acetylation Acylation / physiology* Bacillus subtilis / metabolism Bacterial Proteins / metabolism Escherichia coli / metabolism Geobacillus / metabolism Lysine / metabolism* Protein Processing, Post-Translational / physiology Proteome / metabolism* Rhodothermus / metabolism Succinic Acid / metabolism* Thermus thermophilus / metabolism*
IF 2.609
引用数 15
リソース情報
一般微生物 JCM 9785