RRC ID 50385
著者 Watts BR, Wittmann S, Wery M, Gautier C, Kus K, Birot A, Heo DH, Kilchert C, Morillon A, Vasiljeva L.
タイトル Histone deacetylation promotes transcriptional silencing at facultative heterochromatin.
ジャーナル Nucleic Acids Res
Abstract It is important to accurately regulate the expression of genes involved in development and environmental response. In the fission yeast Schizosaccharomyces pombe, meiotic genes are tightly repressed during vegetative growth. Despite being embedded in heterochromatin these genes are transcribed and believed to be repressed primarily at the level of RNA. However, the mechanism of facultative heterochromatin formation and the interplay with transcription regulation is not understood. We show genome-wide that HDAC-dependent histone deacetylation is a major determinant in transcriptional silencing of facultative heterochromatin domains. Indeed, mutation of class I/II HDACs leads to increased transcription of meiotic genes and accumulation of their mRNAs. Mechanistic dissection of the pho1 gene where, in response to phosphate, transient facultative heterochromatin is established by overlapping lncRNA transcription shows that the Clr3 HDAC contributes to silencing independently of SHREC, but in an lncRNA-dependent manner. We propose that HDACs promote facultative heterochromatin by establishing alternative transcriptional silencing.
巻・号 46(11)
ページ 5426-5440
公開日 2018-6-20
DOI 10.1093/nar/gky232
PII 4956182
PMID 29618061
PMC PMC6009587
MeSH Acid Phosphatase / genetics* Cell Cycle Proteins / metabolism* Chromatin Assembly and Disassembly / genetics Gene Expression Regulation, Fungal / genetics* Heterochromatin / metabolism Histone Deacetylases / metabolism* Histones / metabolism* Meiosis / genetics Protein Processing, Post-Translational / genetics RNA Interference RNA, Long Noncoding / genetics* Schizosaccharomyces / genetics* Schizosaccharomyces pombe Proteins / genetics* Schizosaccharomyces pombe Proteins / metabolism*
IF 11.502
引用数 10
リソース情報
酵母 FY18552(YP344) FY11920(YP381) FY20692(YP390) FY19674(YP383) FY20911(YP713) YP345