RRC ID |
43747
|
著者 |
Hashimoto O, Takagi R, Yanuma F, Doi S, Shindo J, Endo H, Hasegawa Y, Shimasaki S.
|
タイトル |
Identification and characterization of canine growth differentiation factor-9 and its splicing variant.
|
ジャーナル |
Gene
|
Abstract |
Growth differentiation factor-9 (GDF-9), a member of the transforming growth factor-β (TGF-β) superfamily, is expressed exclusively in the oocyte within the ovary and plays essential roles in the ovarian function in mammals. However, a possible involvement of GDF-9 in canine ovarian physiology that has a unique ovulation process among mammals has not been studied. Interestingly, we have isolated two types of cDNA clones generated by an alternative splicing from a canine ovarian total RNA. The predominant long form cDNA shares a common precursor structure with GDF-9s in other species whereas the minor short form cDNA has a 172 amino acid truncation in the proregion. Using a transient expression system, we found that the long form cDNA has a defect in mature protein production whereas the short form cDNA readily produces mature protein. However, mutations at one or two N-glycosylation sites in the mature domain of the short form GDF-9 caused a loss in mature protein production. These results suggest that the prodomain and N-linked glycosylation of the mature domain regulate proper processing and secretion of canine GDF-9. Based on the biological functions of GDF-9, these characteristics of canine GDF-9 could be causatively linked to the unique ovulation process in the Canidae.
|
巻・号 |
499(2)
|
ページ |
266-72
|
公開日 |
2012-5-15
|
DOI |
10.1016/j.gene.2012.03.003
|
PII |
S0378-1119(12)00301-0
|
PMID |
22446043
|
PMC |
PMC3367864
|
MeSH |
Amino Acid Sequence
Animals
Cloning, Molecular
Dogs / metabolism*
Female
Glycosylation
Growth Differentiation Factor 9 / chemistry*
Growth Differentiation Factor 9 / genetics
Growth Differentiation Factor 9 / isolation & purification*
Growth Differentiation Factor 9 / metabolism
Molecular Sequence Data
Ovary / metabolism
Protein Processing, Post-Translational
RNA Splicing
Recombinant Proteins / genetics
Sequence Alignment
|
IF |
2.984
|
引用数 |
4
|
WOS 分野
|
GENETICS & HEREDITY
|
リソース情報 |
ヒト・動物細胞 |
|