RRC ID 50051
著者 Fan W, Lam SM, Xin J, Yang X, Liu Z, Liu Y, Wang Y, Shui G, Huang X.
タイトル Drosophila TRF2 and TAF9 regulate lipid droplet size and phospholipid fatty acid composition.
ジャーナル PLoS Genet
Abstract The general transcription factor TBP (TATA-box binding protein) and its associated factors (TAFs) together form the TFIID complex, which directs transcription initiation. Through RNAi and mutant analysis, we identified a specific TBP family protein, TRF2, and a set of TAFs that regulate lipid droplet (LD) size in the Drosophila larval fat body. Among the three Drosophila TBP genes, trf2, tbp and trf1, only loss of function of trf2 results in increased LD size. Moreover, TRF2 and TAF9 regulate fatty acid composition of several classes of phospholipids. Through RNA profiling, we found that TRF2 and TAF9 affects the transcription of a common set of genes, including peroxisomal fatty acid β-oxidation-related genes that affect phospholipid fatty acid composition. We also found that knockdown of several TRF2 and TAF9 target genes results in large LDs, a phenotype which is similar to that of trf2 mutants. Together, these findings provide new insights into the specific role of the general transcription machinery in lipid homeostasis.
巻・号 13(3)
ページ e1006664
公開日 2017-3-1
DOI 10.1371/journal.pgen.1006664
PII PGENETICS-D-16-02602
PMID 28273089
PMC PMC5362240
MeSH Alleles Amino Acid Motifs Animals Drosophila / genetics* Drosophila / metabolism Drosophila Proteins / metabolism* Fatty Acids / chemistry* Homeostasis Lipids / chemistry* Mutation Oxygen / chemistry Peroxisomes / chemistry Phenotype Phospholipids / chemistry RNA Interference Sequence Analysis, RNA TATA-Binding Protein Associated Factors / metabolism* Telomeric Repeat Binding Protein 2 / metabolism* Transcription Factor TFIID / chemistry Transcription Factor TFIID / metabolism*
IF 5.175
引用数 9
リソース情報
ショウジョウバエ 1773R-1 2617R-1 3006R-1 3394R-2 3571R-1 3589R-2 3656R-1 3700R-2 3714R-2 4073R-3 4080R-2 5270R-2 5554R-1 5958R-1 6012R-1 6163R-1 6331R-2 11474R-1 12926R-1 13334R-1 13434R-1 13897R-1 14629R-1 14974R-1 15632R-1 15822R-1 17224R-3 30059R-1 30152R-1 11976R-1 31102R-1 6572R-1 31904R-2 11156R-1 11275R-1 12036R-1