RRC ID 50622
著者 Otani K, Niwa Y, Suzuki T, Sato N, Sasazawa Y, Dohmae N, Simizu S.
タイトル Regulation of granulocyte colony-stimulating factor receptor-mediated granulocytic differentiation by C-mannosylation.
ジャーナル Biochem Biophys Res Commun
Abstract Granulocyte colony-stimulating factor (G-CSF) receptor (G-CSFR) is a type I cytokine receptor which is involved in hematopoietic cell maturation. G-CSFR has three putative C-mannosylation sites at W253, W318, and W446; however, it is not elucidated whether G-CSFR is C-mannosylated or not. In this study, we first demonstrated that G-CSFR was C-mannosylated at only W318. We also revealed that C-mannosylation of G-CSFR affects G-CSF-dependent downstream signaling through changing ligand binding capability but not cell surface localization. Moreover, C-mannosylation of G-CSFR was functional and regulated granulocytic differentiation in myeloid 32D cells. In conclusion, we found that G-CSFR is C-mannosylated at W318 and that this C-mannosylation has role(s) for myeloid cell differentiation through regulating downstream signaling.
巻・号 498(3)
ページ 466-472
公開日 2018-4-6
DOI 10.1016/j.bbrc.2018.02.210
PII S0006-291X(18)30466-2
PMID 29501745
MeSH Amino Acid Sequence Cell Line, Tumor Granulocytes / cytology* Granulocytes / metabolism HEK293 Cells Humans Leukopoiesis* Mannose / analysis Mannose / metabolism* Receptors, Granulocyte Colony-Stimulating Factor / chemistry Receptors, Granulocyte Colony-Stimulating Factor / metabolism* Signal Transduction
IF 2.985
引用数 3
リソース情報
遺伝子材料 CSII-CMV-MCS-IRES2-Bsd (RDB04385).