| RRC ID |
48006
|
| 著者 |
Shoji K, Suzuki Y, Sugano S, Shimada T, Katsuma S.
|
| タイトル |
Artificial "ping-pong" cascade of PIWI-interacting RNA in silkworm cells.
|
| ジャーナル |
RNA
|
| Abstract |
PIWI-interacting RNAs (piRNAs) play essential roles in the defense system against selfish elements in animal germline cells by cooperating with PIWI proteins. A subset of piRNAs is predicted to be generated via the "ping-pong" cascade, which is mainly controlled by two different PIWI proteins. Here we established a cell-based artificial piRNA production system using a silkworm ovarian cultured cell line that is believed to possess a complete piRNA pathway. In addition, we took advantage of a unique silkworm sex-determining one-to-one ping-pong piRNA pair, which enabled us to precisely monitor the behavior of individual artificial piRNAs. With this novel strategy, we successfully generated artificial piRNAs against endogenous protein-coding genes via the expected back-and-forth traveling mechanism. Furthermore, we detected "primary" piRNAs from the upstream region of the artificial "ping-pong" site in the endogenous gene. This artificial piRNA production system experimentally confirms the existence of the "ping-pong" cascade of piRNAs. Also, this system will enable us to identify the factors involved in both, or each, of the "ping" and "pong" cascades and the sequence features that are required for efficient piRNA production.
|
| 巻・号 |
23(1)
|
| ページ |
86-97
|
| 公開日 |
2017-1-1
|
| DOI |
10.1261/rna.058875.116
|
| PII |
rna.058875.116
|
| PMID |
27777367
|
| PMC |
PMC5159652
|
| MeSH |
Animals
Bombyx / genetics*
Cell Line
Cells, Cultured
Female
Insect Proteins / genetics
Insect Proteins / metabolism
Ovary / cytology*
Ovary / metabolism
RNA, Small Interfering / genetics*
|
| IF |
4.32
|
| 引用数 |
4
|
|
WOS 分野
|
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| リソース情報 |
| カイコ |
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