RRC ID 15316
著者 Shahriari M, Richter K, Keshavaiah C, Sabovljevic A, Huelskamp M, Schellmann S.
タイトル The Arabidopsis ESCRT protein-protein interaction network.
ジャーナル Plant Mol Biol
Abstract In yeast, endosomal sorting of monoubiquitylated transmembrane proteins is performed by a subset of the 19 "class E vacuolar protein sorting" proteins. The core machinery consists of 11 proteins that are organised in three complexes termed ESCRT I-III (endosomal sorting complex required for transport I-III) and is conserved in eukaryotic cells. While the pathway is well understood in yeast and animals, the plant ESCRT system is largely unexplored. At least one sequence homolog for each ESCRT component can be found in the Arabidopsis genome. Generally, sequence conservation between yeast/animals and the Arabidopsis proteins is low. To understand details about participating proteins and complex organization we have performed a systematic pairwise yeast two hybrid analysis of all Arabidopsis proteins showing homology to the ESCRT core machinery. Positive interactions were validated using bimolecular fluorescence complementation. In our experiments, most putative ESCRT components exhibited interactions with other ESCRT components that could be shown to occur on endosomes suggesting that despite their low homology to their yeast and animal counterparts they represent functional components of the plant ESCRT pathway.
巻・号 76(1-2)
ページ 85-96
公開日 2011-5-1
DOI 10.1007/s11103-011-9770-4
PMID 21442383
MeSH Animals Arabidopsis / cytology Arabidopsis / genetics Arabidopsis / metabolism* Arabidopsis Proteins / genetics Arabidopsis Proteins / metabolism* Computer Simulation Endosomal Sorting Complexes Required for Transport / metabolism* Luminescent Proteins / genetics Luminescent Proteins / metabolism Microscopy, Confocal Models, Biological Protein Binding Protein Interaction Mapping / methods* Protoplasts / metabolism Recombinant Fusion Proteins / genetics Recombinant Fusion Proteins / metabolism Saccharomyces cerevisiae / genetics Signal Transduction Two-Hybrid System Techniques
IF 3.302
引用数 31
WOS 分野 PLANT SCIENCES BIOCHEMISTRY & MOLECULAR BIOLOGY
リソース情報
シロイヌナズナ / 植物培養細胞・遺伝子 pda04818 pda09962 pda06592 pda08063 pda03672 pda11165 pda02568 pda02482 pda01247 pda02381 pdx96416 pdx96789 pdy35572 pda02252 pdx36377 pdx95869 pdx99743