RRC ID |
6277
|
著者 |
Jia F, Gampala SS, Mittal A, Luo Q, Rock CD.
|
タイトル |
Cre-lox univector acceptor vectors for functional screening in protoplasts: analysis of Arabidopsis donor cDNAs encoding ABSCISIC ACID INSENSITIVE1-like protein phosphatases.
|
ジャーナル |
Plant Mol Biol
|
Abstract |
The 14,200 available full length Arabidopsis thaliana cDNAs in the universal plasmid system (UPS) donor vector pUNI51 should be applied broadly and efficiently to leverage a "functional map-space" of homologous plant genes. We have engineered Cre-lox UPS host acceptor vectors (pCR701- 705) with N-terminal epitope tags in frame with the loxH site and downstream from the maize Ubiquitin promoter for use in transient protoplast expression assays and particle bombardment transformation of monocots. As an example of the utility of these vectors, we recombined them with several Arabidopsis cDNAs encoding Ser/Thr protein phosphatase type 2C (PP2Cs) known from genetic studies or predicted by hierarchical clustering meta-analysis to be involved in ABA and stress responses. Our functional results in Zea mays mesophyll protoplasts on ABA-inducible expression effects on the Late Embryogenesis Abundant promoter ProEm:GUS reporter were consistent with predictions and resulted in identification of novel activities of some PP2Cs. Deployment of these vectors can facilitate functional genomics and proteomics and identification of novel gene activities.
|
巻・号 |
70(6)
|
ページ |
693-708
|
公開日 |
2009-8-1
|
DOI |
10.1007/s11103-009-9502-1
|
PMID |
19499346
|
PMC |
PMC2755202
|
MeSH |
Abscisic Acid / metabolism
Arabidopsis / enzymology*
Arabidopsis / genetics*
Arabidopsis Proteins / genetics*
Arabidopsis Proteins / metabolism*
DNA, Complementary / genetics
DNA, Plant / genetics*
Gene Expression Profiling
Genetic Vectors
Phosphoprotein Phosphatases / genetics*
Phosphoprotein Phosphatases / metabolism*
Phylogeny
Plants, Genetically Modified
Protein Phosphatase 2C
Protoplasts / metabolism
Transformation, Genetic
Zea mays / genetics
Zea mays / metabolism
|
IF |
3.302
|
引用数 |
6
|
WOS 分野
|
BIOCHEMISTRY & MOLECULAR BIOLOGY
PLANT SCIENCES
|
リソース情報 |
シロイヌナズナ / 植物培養細胞・遺伝子 |
pda11685
pda11076
pdy22231
pdz88907
pda19470 |