RRC ID |
711
|
著者 |
Morimoto R, Kosugi T, Nakamura C, Takumi S.
|
タイトル |
Intragenic diversity and functional conservation of the three homoeologous loci of the KN1-type homeobox gene Wknox1 in common wheat.
|
ジャーナル |
Plant Mol Biol
|
Abstract |
Common wheat represents a typical allohexaploid species and provides a good experimental system for studying genomic alterations associated with allopolyploidization. We studied three homoeologous loci of wheat Wknox1 gene, which is highly homologous to the knotted1 (kn1)-like homeobox (KNOX) genes functioning at shoot apical meristems (SAM). Wknox1 transcripts were detected in SAM, and its overexpression caused abnormal leaf morphology with occasional ectopic leaves in transgenic tobacco plants. A comparative study of the three Wknox1 genomic sequences revealed accumulation of a large number of mutations including insertions and deletions, particularly in the fourth intron and the 5'-upstream region. Some structural mutations including MITE-insertions were allocated in the evolutionary lineage of the wheat genome, suggesting that they occurred at all stages of wheat evolution. A mutation rate was the highest in the Wknox1b locus, which is consistent with the known highest degree of differentiation in the B genome. Despite the structural differentiation, all three Wknox1 homoeologs showed an identical expression pattern in wheat and their promoter regions induced the conserved expression pattern in transgenic tobacco plants. A potential of the intragenic diversity in homoeologs of essential genes as a tool for studying the genome evolution associated with allopolyploidization was discussed.
|
巻・号 |
57(6)
|
ページ |
907-24
|
公開日 |
2005-4-1
|
DOI |
10.1007/s11103-005-3247-2
|
PMID |
15952073
|
MeSH |
5' Flanking Region / genetics
Base Sequence
Cloning, Molecular
DNA Transposable Elements / genetics
DNA, Plant / chemistry
DNA, Plant / genetics*
Evolution, Molecular
Exons / genetics
Gene Expression Profiling
Genes, Plant / genetics
Genetic Variation
Genome, Plant
Glucuronidase / genetics
Glucuronidase / metabolism
Homeodomain Proteins / genetics*
Homeodomain Proteins / physiology
Introns / genetics
Molecular Sequence Data
Mutagenesis, Insertional
Mutation
Plant Proteins / genetics*
Plant Proteins / physiology
Plants, Genetically Modified
Polymorphism, Single Nucleotide
Polyploidy
Protein Isoforms / genetics
Recombinant Fusion Proteins / genetics
Recombinant Fusion Proteins / metabolism
Sequence Analysis, DNA
Sequence Deletion
Sequence Homology, Nucleic Acid
Triticum / genetics*
Triticum / physiology
|
IF |
3.302
|
引用数 |
20
|
WOS 分野
|
PLANT SCIENCES
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
リソース情報 |
コムギ |
KU-199-11
KU-105
KU-3636
KU-101-2
KU-101-3
KU-103
KU-1-2
KU-1-3
KU-2-4
KU-2-5
KU-3-2
KU-3-3
KU-4-5
KU-4-6
KU-4-7
KU-5-2
KU-5-3
KU-20-1
KU-20-3
KU-20-6
etc |