| RRC ID |
11310
|
| 著者 |
Tsuchida K, Yokoyama T, Sakudoh T, Katagiri C, Tsurumaru S, Takada N, Fujimoto H, Ziegler R, Iwano H, Hamano K, Yaginuma T.
|
| タイトル |
Apolipophorin-III expression and low density lipophorin formation during embryonic development of the silkworm, Bombyx mori.
|
| ジャーナル |
Comp Biochem Physiol B Biochem Mol Biol
|
| Abstract |
We examined the expression of apolipophorin-III (apoLp-III) during embryonic development of the silkworm Bombyx mori. ApoLp-III mRNA was first expressed 24h after oviposition, which corresponds to the time of germ band formation. The amount of apoLp-III in the eggs increased from day 2, peaked on day 4, and then gradually decreased until hatching (on day 9.5). ApoLp-III was apparently synthesized during early embryogenesis, as radioactive amino acids were incorporated into newly synthesized apoLp-III in three-day-old eggs. Moreover, radioactive apoLp-III was found only in the embryo and not in the extraembryonic tissue. KBr density gradient ultracentrifugation of egg homogenates showed that apoLp-III was associated with low-density lipophorin (LDLp). These results suggest that LDLp is required for the delivery of lipids for organogenesis during embryogenesis.
|
| 巻・号 |
155(4)
|
| ページ |
363-70
|
| 公開日 |
2010-4-1
|
| DOI |
10.1016/j.cbpb.2009.12.006
|
| PII |
S1096-4959(10)00006-0
|
| PMID |
20079870
|
| MeSH |
Animals
Apolipoproteins / genetics
Apolipoproteins / metabolism*
Bombyx / embryology*
Bombyx / genetics
Bombyx / metabolism
Electrophoresis, Polyacrylamide Gel
Embryo, Nonmammalian / metabolism*
Embryonic Development
Female
Insect Proteins / genetics
Insect Proteins / metabolism*
Lipoproteins / genetics
Lipoproteins / metabolism*
|
| IF |
2.219
|
| 引用数 |
8
|
|
WOS 分野
|
ZOOLOGY
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| リソース情報 |
| カイコ |
NA |
