RRC ID 579
著者 Bao Y, Yamano Y, Morishima I.
タイトル A novel lebocin-like gene from eri-silkworm, Samia cynthia ricini, that does not encode the antibacterial peptide lebocin.
ジャーナル Comp Biochem Physiol B Biochem Mol Biol
Abstract A cDNA clone with homology to lebocin gene was isolated from fat body of immunized Samia cynthia ricini larvae. The cDNA has an open reading frame encoding 162 amino acid residues. The deduced amino acid sequence shows significant homology to lebocin precursor proteins from Bombyx mori and Trichoplusia ni only in the "prosegment" region, but no homology to mature lebocin, a proline-rich antibacterial peptide, indicating the protein is not a precursor for lebocin antibacterial peptide. Northern analysis indicates that transcript of the lebocin-like gene is not detected in any tissues of naive larvae, but induced mainly in fat body after injection of the larvae with bacterial cells or cell wall components, such as peptidoglycan.
巻・号 140(1)
ページ 127-31
公開日 2005-1-1
DOI 10.1016/j.cbpc.2004.09.022
PII S1096-4959(04)00305-7
PMID 15621517
MeSH Amino Acid Sequence Animals Anti-Infective Agents / chemistry* Antimicrobial Cationic Peptides / pharmacology Bacteria / pathogenicity Base Sequence Blotting, Northern Bombyx / chemistry* Cloning, Molecular DNA, Complementary / genetics DNA, Complementary / metabolism Fat Body Insect Proteins / genetics Insect Proteins / isolation & purification Insect Proteins / pharmacology* Larva / drug effects Larva / microbiology Molecular Sequence Data Open Reading Frames Peptidoglycan / pharmacology Sequence Homology, Amino Acid
IF 2.219
引用数 20
WOS 分野 ZOOLOGY BIOCHEMISTRY & MOLECULAR BIOLOGY
リソース情報
カイコ Samia cynthia ricini