RRC ID 51132
著者 Cao T, Mu S, Lu C, Zhao S, Li D, Yan K, Xiang W, Liu C.
タイトル Streptomyces amphotericinicus sp. nov., an amphotericin-producing actinomycete isolated from the head of an ant (Camponotus japonicus Mayr).
ジャーナル Int J Syst Evol Microbiol
Abstract A novel actinomycete, designated strain 1H-SSA8T, was isolated from the head of an ant (Camponotus japonicus Mayr) and was found to produce amphotericin. A polyphasic approach was employed to determine the status of strain 1H-SSA8T. Morphological and chemotaxonomic characteristics were consistent with those of members of the genus Streptomyces. The menaquinones detected were MK-9(H6), MK-9(H8) and MK-9(H4). The phospholipid profile consisted of diphosphatidylglycerol, phosphatidylmethylethanolamine, phosphatidylethanolamine and phosphatidylinositol mannoside. The major fatty acids were identified as iso-C16 : 0, C16 : 0, C15 : 0 and anteiso-C15 : 0. Analysis of the 16S rRNA gene sequence showed that strain 1H-SSA8T belongs to the genus Streptomyces with high sequence similarity to Streptomyces ramulosus NRRL B-2714T (99.2 %). Two tree-making algorithms based on 16S rRNA gene sequences showed that the isolate formed a phyletic line with Streptomyces himastatinicus ATCC 53653T (98.7 %). The MLSA utilizing partial sequences of the housekeeping genes (atpD, gyrB, recA, rpoB and trpB) also supported the position. However, evolutionary distances were higher than the 0.007 MLSA evolutionary distance threshold proposed for species-level relatedness. Moreover, the low level of DNA-DNA relatedness and phenotypic differences allowed the novel isolate to be differentiated from its most closely related strain S. ramulosus NRRL B-2714T and strain S. himastatinicus ATCC 53653T. It is concluded that the organism can be classified as representing a novel species of the genus Streptomyces, for which the name Streptomyces amphotericinicus sp. nov. is proposed. The type strain is 1H-SSA8T (=CGMCC 4.7350T=DSM 103128T).
巻・号 67(12)
ページ 4967-4973
公開日 2017-12-1
DOI 10.1099/ijsem.0.002382
PMID 29034849
MeSH Amphotericin B / biosynthesis* Animals Ants / microbiology* Bacterial Typing Techniques Base Composition China DNA, Bacterial / genetics Fatty Acids / chemistry Phospholipids / chemistry Phylogeny* RNA, Ribosomal, 16S / genetics Sequence Analysis, DNA Streptomyces / classification* Streptomyces / genetics Streptomyces / isolation & purification Vitamin K 2 / analogs & derivatives Vitamin K 2 / chemistry
IF 2.415
引用数 2
リソース情報
一般微生物 JCM 4604