RRC ID 31977
著者 Vuong LT, Mukhopadhyay B, Choi KW.
タイトル Kinesin-II recruits Armadillo and Dishevelled for Wingless signaling in Drosophila.
ジャーナル Development
Abstract Wingless (Wg)/Wnt signaling is fundamental in metazoan development. Armadillo (Arm)/β-catenin and Dishevelled (Dsh) are key components of Wnt signal transduction. Recent studies suggest that intracellular trafficking of Wnt signaling components is important, but underlying mechanisms are not well known. Here, we show that Klp64D, the Drosophila homolog of Kif3A kinesin II subunit, is required for Wg signaling by regulating Arm during wing development. Mutations in klp64D or RNAi cause wing notching and loss of Wg target gene expression. The wing notching phenotype by Klp64D knockdown is suppressed by activated Arm but not by Dsh, suggesting that Klp64D is required for Arm function. Furthermore, klp64D and arm mutants show synergistic genetic interaction. Consistent with this genetic interaction, Klp64D directly binds to the Arm repeat domain of Arm and can recruit Dsh in the presence of Arm. Overexpression of Klp64D mutated in the motor domain causes dominant wing notching, indicating the importance of the motor activity. Klp64D shows subcellular localization to intracellular vesicles overlapping with Arm and Dsh. In klp64D mutants, Arm is abnormally accumulated in vesicular structures including Golgi, suggesting that intracellular trafficking of Arm is affected. Human KIF3A can also bind β-catenin and rescue klp64D RNAi phenotypes. Taken together, we propose that Klp64D is essential for Wg signaling by trafficking of Arm via the formation of a conserved complex with Arm.
巻・号 141(16)
ページ 3222-32
公開日 2014-8-1
DOI 10.1242/dev.106229
PII dev.106229
PMID 25063455
MeSH Adaptor Proteins, Signal Transducing / physiology* Amino Acid Sequence Animals Armadillo Domain Proteins / physiology* Body Patterning Dishevelled Proteins Drosophila Proteins / genetics Drosophila Proteins / physiology* Drosophila melanogaster / embryology Drosophila melanogaster / genetics Gene Expression Profiling Gene Expression Regulation, Developmental Golgi Apparatus / metabolism Humans Kinesins / genetics Kinesins / metabolism Kinesins / physiology* Molecular Sequence Data Mutation Phenotype Phosphoproteins / physiology* RNA Interference Sequence Homology, Amino Acid Signal Transduction Transcription Factors / physiology* Wings, Animal / embryology beta Catenin / metabolism
IF 5.611
引用数 8
WOS 分野 DEVELOPMENTAL BIOLOGY
リソース情報
ショウジョウバエ 10642R-1 10642R-2