RRC ID 36903
著者 Takeuchi T, Shimakawa G, Tamura M, Yokosawa H, Arata Y.
タイトル ISG15 regulates RANKL-induced osteoclastogenic differentiation of RAW264 cells.
ジャーナル Biol Pharm Bull
Abstract Interferon-stimulated gene 15 kDa (ISG15) is a protein upregulated by interferon-β that negatively regulates osteoclastogenesis. We investigated the role of ISG15 in receptor activator of nuclear factor-κB ligand (RANKL)-induced osteoclastogenic differentiation of murine RAW264 cells. RANKL stimulation induced ISG15 expression in RAW264 cells at both the mRNA and protein levels. Overexpression of ISG15 in RAW264 cells resulted in suppression of cell fusion in RANKL-stimulated cells as well as the reduced expression of ATP6v0d2, a gene essential for cell fusion in osteoclastogenic differentiation. These results suggest that ISG15 suppresses RANKL-induced osteoclastogenesis, at least in part, through inhibition of ATP6v0d2 expression.
巻・号 38(3)
ページ 482-6
公開日 2015-1-1
DOI 10.1248/bpb.b14-00410
PMID 25757932
MeSH Animals Bone Marrow Cells Bone Resorption / metabolism Cell Differentiation* Cell Line Cytokines / genetics Cytokines / metabolism* Interferon-beta / metabolism* Macrophages / metabolism Mice NF-kappa B / metabolism NFATC Transcription Factors / metabolism Osteoclasts / physiology* RANK Ligand / metabolism* RNA, Messenger / metabolism Ubiquitins / genetics Ubiquitins / metabolism Vacuolar Proton-Translocating ATPases / metabolism
IF 1.863
引用数 4
WOS 分野 PHARMACOLOGY & PHARMACY
リソース情報
ヒト・動物細胞 RAW 264(RCB0535)