RRC ID 41309
著者 Oshima Y, Seki K, Shibuya M, Naka Y, Yokoyama T, Sato A.
タイトル Soluble Human Intestinal Lactoferrin Receptor: Ca(2+)-Dependent Binding to Sepharose-Based Matrices.
ジャーナル Biol Pharm Bull
Abstract A soluble form of human intestinal lactoferrin receptor (shLFR) is identical to human intelectin-1 (hITLN-1), a galactofuranose-binding protein that acts as a host defense against invading pathogenic microorganisms. We found that recombinant shLFR, expressed in mammalian cells (CHO DG44, COS-1, and RK13), binds tightly to Sepharose 4 Fast Flow (FF)-based matrices in a Ca(2+)-dependent manner. This binding of shLFR to Sepharose 4 FF-based matrices was inhibited by excess D-galactose, but not by D-glucose, suggesting that shLFR recognizes repeating units of α-1,6-linked D-galactose in Sepharose 4 FF. Furthermore, shLFR could bind to both Sepharose 4B- and Sepharose 6B-based matrices that were not crosslinked in a similar manner as to Sepharose 4 FF-based matrices. Therefore, shLFR (hITLN-1) binds to Sepharose-based matrices in a Ca(2+)-dependent manner. This binding property is most likely related to the ability, as host defense lectins, to recognize sepharose (agarobiose)-like structures present on the surface of invading pathogenic microorganisms.
巻・号 39(3)
ページ 435-9
公開日 2016-1-1
DOI 10.1248/bpb.b15-00643
PMID 26934934
MeSH Animals CHO Cells COS Cells Calcium / metabolism* Cell Line Chlorocebus aethiops Cricetulus Humans Intestines Protein Binding Rabbits Receptors, Cell Surface / genetics Receptors, Cell Surface / metabolism* Recombinant Proteins / metabolism Sepharose / metabolism*
IF 1.863
引用数 3
WOS 分野 PHARMACOLOGY & PHARMACY
リソース情報
遺伝子材料 pOptiVEC/LFRF (RDB14246) pOptiVEC/LFRF (RDB14246).
ヒト・動物細胞 RK13(RCB0183)