RRC ID 21122
著者 Weichert A, Brinkmann C, Komarova NY, Dietrich D, Thor K, Meier S, Suter Grotemeyer M, Rentsch D.
タイトル AtPTR4 and AtPTR6 are differentially expressed, tonoplast-localized members of the peptide transporter/nitrate transporter 1 (PTR/NRT1) family.
ジャーナル Planta
Abstract Members of the peptide transporter/nitrate transporter 1 (PTR/NRT1) family in plants transport a variety of substrates like nitrate, di- and tripepetides, auxin and carboxylates. We isolated two members of this family from Arabidopsis, AtPTR4 and AtPTR6, which are highly homologous to the characterized di- and tripeptide transporters AtPTR1, AtPTR2 and AtPTR5. All known substrates of members of the PTR/NRT1 family were tested using heterologous expression in Saccharomyces cerevisiae mutants and oocytes of Xenopus laevis, but none could be identified as substrate of AtPTR4 or AtPTR6. AtPTR4 and AtPTR6 show distinct expression patterns, while AtPTR4 is expressed in the vasculature of the plants, AtPTR6 is highly expressed in pollen and during senescence. Phylogenetic analyses revealed that AtPTR2, 4 and 6 belong to one clade of subgoup II, whereas AtPTR1 and 5 are found in a second clade. Like AtPTR2, AtPTR4-GFP and AtPTR6-GFP fusion proteins are localized at the tonoplast. Vacuolar localization was corroborated by co-localization of AtPTR2-YFP with the tonoplast marker protein GFP-AtTIP2;1 and AtTIP1;1-GFP. This indicates that the two clades reflect different intracellular localization at the tonoplast (AtPTR2, 4, 6) and plasma membrane (AtPTR1, 5), respectively.
巻・号 235(2)
ページ 311-23
公開日 2012-2-1
DOI 10.1007/s00425-011-1508-7
PMID 21904872
MeSH Animals Anion Transport Proteins / classification Anion Transport Proteins / genetics Anion Transport Proteins / metabolism* Arabidopsis / classification Arabidopsis / genetics Arabidopsis / metabolism* Arabidopsis Proteins / genetics Arabidopsis Proteins / metabolism Axenic Culture Cell Membrane / genetics Cell Membrane / metabolism Cloning, Molecular Gene Expression Regulation, Plant* Green Fluorescent Proteins / genetics Green Fluorescent Proteins / metabolism Intracellular Membranes / metabolism* Membrane Transport Proteins / genetics Membrane Transport Proteins / metabolism Microscopy, Confocal Oocytes / cytology Oocytes / metabolism Open Reading Frames Phylogeny Plant Proteins / classification Plant Proteins / genetics Plant Proteins / metabolism* Pollen / genetics Pollen / metabolism Protoplasts / cytology Protoplasts / metabolism RNA, Plant / genetics RNA, Plant / metabolism Recombinant Fusion Proteins / genetics Recombinant Fusion Proteins / metabolism Saccharomyces cerevisiae / genetics Saccharomyces cerevisiae / metabolism Vacuoles / metabolism Xenopus laevis / genetics Xenopus laevis / metabolism
IF 3.39
引用数 32
WOS 分野 PLANT SCIENCES
リソース情報
シロイヌナズナ / 植物培養細胞・遺伝子 pda05229