RRC ID 28106
著者 Goss CA, Brockmann DJ, Bushoven JT, Roberts AW.
タイトル A CELLULOSE SYNTHASE (CESA) gene essential for gametophore morphogenesis in the moss Physcomitrella patens.
ジャーナル Planta
Abstract In seed plants, different groups of orthologous genes encode the CELLULOSE SYNTHASE (CESA) proteins that are responsible for cellulose biosynthesis in primary and secondary cell walls. The seven CESA sequences of the moss Physcomitrella patens (Hedw.) B. S. G. form a monophyletic sister group to seed plant CESAs, consistent with independent CESA diversification and specialization in moss and seed plant lines. The role of PpCESA5 in the development of P. patens was investigated by targeted mutagenesis. The cesa5 knockout lines were tested for cellulose deficiency using carbohydrate-binding module affinity cytochemistry and the morphology of the leafy gametophores was analyzed by 3D reconstruction of confocal images. No defects were identified in the development of the filamentous protonema or in production of bud initials that normally give rise to the leafy gametophores. However, the gametophore buds were cellulose deficient and defects in subsequent cell expansion, cytokinesis, and leaf initiation resulted in the formation of irregular cell clumps instead of leafy shoots. Analysis of the cesa5 knockout phenotype indicates that a biophysical model of organogenesis can be extended to the moss gametophore shoot apical meristem.
巻・号 235(6)
ページ 1355-67
公開日 2012-6-1
DOI 10.1007/s00425-011-1579-5
PMID 22215046
MeSH Bryopsida / enzymology* Bryopsida / genetics* Bryopsida / growth & development Cellulose / metabolism Gene Expression Regulation, Enzymologic Gene Expression Regulation, Plant Gene Knockout Techniques Genes, Essential / genetics* Genes, Plant / genetics* Genetic Complementation Test Genetic Vectors / genetics Genotype Germ Cells, Plant / cytology Germ Cells, Plant / growth & development* Germ Cells, Plant / metabolism Glucosyltransferases / genetics* Glucosyltransferases / metabolism Microscopy, Confocal Morphogenesis / genetics* Reproducibility of Results Restriction Mapping Reverse Transcriptase Polymerase Chain Reaction Transformation, Genetic
IF 3.39
引用数 20
WOS 分野 PLANT SCIENCES
リソース情報
シロイヌナズナ / 植物培養細胞・遺伝子 pdp24095