RRC ID 33160
著者 Iwase A, Mita K, Nonaka S, Ikeuchi M, Koizuka C, Ohnuma M, Ezura H, Imamura J, Sugimoto K.
タイトル WIND1-based acquisition of regeneration competency in Arabidopsis and rapeseed.
ジャーナル J Plant Res
Abstract Callus formation and de novo organogenesis often occur in the wounded tissues of plants. Although this regenerative capacity of plant cells has been utilized for many years, molecular basis for the wound-induced acquisition of regeneration competency is yet to be elucidated. Here we find that wounding treatment is essential for shoot regeneration from roots in the conventional tissue culture of Arabidopsis thaliana. Furthermore, we show that an AP2/ERF transcription factor WOUND INDUCED DEDIFFERENTIATION1 (WIND1) plays a pivotal role for the acquisition of regeneration competency in the culture system. Ectopic expression of WIND1 can bypass both wounding and auxin pre-treatment and increase de novo shoot regeneration from root explants cultured on shoot-regeneration promoting media. In Brassica napus, activation of Arabidopsis WIND1 also greatly enhances de novo shoot regeneration, further corroborating the role of WIND1 in conferring cellular regenerative capacity. Our data also show that sequential activation of WIND1 and an embryonic regulator LEAFY COTYLEDON2 enhances generation of embryonic callus, suggesting that combining WIND1 with other transcription factors promote efficient and organ-specific regeneration. Our findings in the model plant and crop plant point to a possible way to efficiently induce callus formation and regeneration by utilizing transcription factors as a molecular switch.
巻・号 128(3)
ページ 389-97
公開日 2015-5-1
DOI 10.1007/s10265-015-0714-y
PMID 25810222
MeSH Arabidopsis / genetics Arabidopsis / physiology* Arabidopsis Proteins / genetics Arabidopsis Proteins / metabolism* Brassica napus / genetics Brassica napus / physiology* Indoleacetic Acids / metabolism Organ Specificity Plant Growth Regulators / metabolism* Plant Leaves / genetics Plant Leaves / physiology Plant Proteins / genetics Plant Proteins / metabolism* Plant Roots / genetics Plant Roots / physiology Plant Shoots / genetics Plant Shoots / physiology Plant Somatic Embryogenesis Techniques Plants, Genetically Modified Regeneration Transcription Factors / genetics Transcription Factors / metabolism*
IF 2.185
引用数 27
WOS 分野 PLANT SCIENCES
リソース情報
トマト TOMJPF00001