RRC ID 35163
著者 Huang YX, Yin YG, Sanuki A, Fukuda N, Ezura H, Matsukura C.
タイトル Phosphoenolpyruvate carboxykinase (PEPCK) deficiency affects the germination, growth and fruit sugar content in tomato (Solanum lycopersicum L.).
ジャーナル Plant Physiol Biochem
Abstract Phosphoenolpyruvate carboxykinase (PEPCK) is a key regulatory enzyme and is utilized in the gluconeogenesis pathway in plants. Although, its catalytic and regulatory properties are quite well understood, there are uncertainties regarding its physiological role in many plants tissues such as the flesh of developing fruits. To further understand the function of PEPCK in fruits and other tissues, RNAi transgenic tomato plants in which SlPEPCK transcription was down-regulated by either CaMV 35S constitutive promoter or the fruit-specific E8 promoter were generated and characterized on the basis of their phenotypic and metabolic aspects. In the PEPCK-deficient lines, prominent growth suppression of germinated seedlings was observed and other vegetative suppression appeared during the early stage of plant growth in the 35S promoter-driven lines. In particular, root elongation was most obviously suppressed in the germinated seedlings, indicating that the gluconeogenesis pathway is involved in the root growth of seedlings. Regarding the primary metabolism in fruit, the soluble sugar content tended to decrease, whereas the malate content tended to increase in ripening fruits of the RNAi lines compared with the wild type. These results indicate that activation of the gluconeogenesis pathway from organic acids to sugars occurs during ripening but is suppressed by the knocking down of the PEPCK gene, suggesting that PEPCK participates in determining the sugar/acid ratio in ripening fruit.
巻・号 96
ページ 417-25
公開日 2015-11-1
DOI 10.1016/j.plaphy.2015.08.021
PII S0981-9428(15)30101-7
PMID 26381194
MeSH Carbohydrate Metabolism* Gene Expression Regulation, Enzymologic Gene Expression Regulation, Plant Germination / physiology* Phosphoenolpyruvate Carboxykinase (ATP) / genetics Phosphoenolpyruvate Carboxykinase (ATP) / metabolism Phosphoenolpyruvate Carboxykinase (ATP) / physiology* Plants, Genetically Modified Solanum lycopersicum / enzymology* Solanum lycopersicum / growth & development Solanum lycopersicum / metabolism Solanum lycopersicum / physiology Transcription, Genetic
IF 3.72
引用数 12
WOS 分野 PLANT SCIENCES
リソース情報
トマト TOMJPF00001