RRC ID 36770
著者 Kim YI, Mohri S, Hirai S, Lin S, Goto T, Ohyane C, Sakamoto T, Takahashi H, Shibata D, Takahashi N, Kawada T.
タイトル Tomato extract suppresses the production of proinflammatory mediators induced by interaction between adipocytes and macrophages.
ジャーナル Biosci Biotechnol Biochem
Abstract Obese adipose tissue is characterized by enhanced macrophage infiltration. A loop involving monocyte chemoattractant protein-1 (MCP-1) and tumor necrosis factor-α (TNFα) between adipocytes and macrophages establishes a vicious cycle that augments inflammatory changes and insulin resistance in obese adipose tissue. Tomatoes, one of the most popular crops worldwide, contain many beneficial phytochemicals that improve obesity-related diseases such as diabetes. Some of them have also been reported to have anti-inflammatory properties. In this study, we focused on the potential protective effects of phytochemicals in tomatoes on inflammation. We screened fractions of tomato extract using nitric oxide (NO) assay in lipopolysaccharide (LPS)-stimulated RAW264 macrophages. One fraction, RF52, significantly inhibited NO production in LPS-stimulated RAW264 macrophages. Furthermore, RF52 significantly decreased MCP-1 and TNFα productions. The coculture of 3T3-L1 adipocytes and RAW264 macrophages markedly enhanced MCP-1, TNFα, and NO productions compared with the control cultures; however, the treatment with RF52 inhibited the production of these proinflammatory mediators. These results suggest that RF52 from tomatoes may have the potential to suppress inflammation by inhibiting the production of NO or proinflammatory cytokines during the interaction between adipocytes and macrophages.
巻・号 79(1)
ページ 82-7
公開日 2015-1-1
DOI 10.1080/09168451.2014.962472
PMID 25603813
MeSH 3T3-L1 Cells Adipocytes / cytology Adipocytes / drug effects* Adipocytes / metabolism Animals Anti-Inflammatory Agents / chemistry Anti-Inflammatory Agents / pharmacology* Cell Communication Cell Differentiation Cell Line Chemokine CCL2 / antagonists & inhibitors Chemokine CCL2 / biosynthesis Coculture Techniques Lipopolysaccharides / antagonists & inhibitors Lipopolysaccharides / pharmacology Macrophage Activation Macrophages / cytology Macrophages / drug effects* Macrophages / metabolism Mice Nitric Oxide / antagonists & inhibitors Nitric Oxide / biosynthesis Phytochemicals / pharmacology* Plant Extracts / chemistry Plant Extracts / pharmacology* Solanum lycopersicum / chemistry* Tumor Necrosis Factor-alpha / antagonists & inhibitors Tumor Necrosis Factor-alpha / biosynthesis
IF 1.516
引用数 6
WOS 分野 FOOD SCIENCE & TECHNOLOGY BIOTECHNOLOGY & APPLIED MICROBIOLOGY CHEMISTRY, APPLIED BIOCHEMISTRY & MOLECULAR BIOLOGY
リソース情報
ヒト・動物細胞 RAW 264(RCB0535)