RRC ID 48058
著者 Matsuo Y, Kawamukai M.
タイトル cAMP-dependent protein kinase involves calcium tolerance through the regulation of Prz1 in Schizosaccharomyces pombe.
ジャーナル Biosci Biotechnol Biochem
Abstract The cAMP-dependent protein kinase Pka1 is known as a regulator of glycogenesis, meiosis, and stress responses in Schizosaccharomyces pombe. We demonstrated that Pka1 is responsible for calcium tolerance. Loss of functional components of the PKA pathway such as Git3, Gpa2, Cyr1, and Pka1 yields a CaCl2-sensitive phenotype, while loss of Cgs1, a regulatory subunit of PKA, results in CaCl2 tolerance. Cytoplasmic distribution of Cgs1 and Pka1 is increased by the addition of CaCl2, suggesting that CaCl2 induces dissociation of Cgs1 and Pka1. The expression of Prz1, a transcriptional regulator in calcium homeostasis, is elevated in a pka1∆ strain and in a wild type strain under glucose-limited conditions. Accordingly, higher expression of Prz1 in the wild type strain results in a CaCl2-sensitive phenotype. These findings suggest that Pka1 is essential for tolerance to exogenous CaCl2, probably because the expression level of Prz1 needs to be properly regulated by Pka1.
巻・号 81(2)
ページ 231-241
公開日 2017-2-1
DOI 10.1080/09168451.2016.1246171
PMID 27756188
MeSH Calcium Chloride / pharmacology* Cyclic AMP-Dependent Protein Kinase Catalytic Subunits / metabolism Cyclic AMP-Dependent Protein Kinases / deficiency Cyclic AMP-Dependent Protein Kinases / genetics Cyclic AMP-Dependent Protein Kinases / metabolism* Enzyme Activation / drug effects Gene Deletion Homeostasis / drug effects Phenotype Protein Transport / drug effects Schizosaccharomyces / drug effects* Schizosaccharomyces / enzymology Schizosaccharomyces / genetics Schizosaccharomyces / metabolism* Schizosaccharomyces pombe Proteins / genetics Schizosaccharomyces pombe Proteins / metabolism* Transcription Factors / genetics Transcription Factors / metabolism*
IF 1.516
引用数 4
WOS 分野 FOOD SCIENCE & TECHNOLOGY BIOTECHNOLOGY & APPLIED MICROBIOLOGY BIOCHEMISTRY & MOLECULAR BIOLOGY CHEMISTRY, APPLIED
リソース情報
酵母 FY17838