RRC ID 30792
著者 Ito K, Ren J, Fujita T.
タイトル Conserved function of Rho-related Rop/RAC GTPase signaling in regulation of cell polarity in Physcomitrella patens.
ジャーナル Gene
Abstract Cell polarity is fundamentally important to growth and development in higher plants, from pollen tubes to root hairs. Basal land plants (mosses and ferns) also have cell polarity, developing protonemal apical cells that show polar tip growth. Flowering plants have a distinct group of Rho GTPases that regulate polarity in polarized cell growth. Rop/RAC signaling module components have been identified in non-flowering plants, but their roles remain unclear. To understand the importance and evolution of Rop/RAC signaling in polarity regulation in land plants, we examined the functions of PpRop and PpRopGEF in protonemal apical cells of the moss Physcomitrella patens. Inducible overexpression of PpRop2 or PpRopGEF3 caused depolarized growth of tip-growing apical cells. PpRop2 overexpression also caused aberrant cross wall formation. Fluorescent protein-tagged PpRop2 localized to the plasma membrane, including the cross wall membrane, and fluorescent-tagged PpRopGEF3 showed polarized localization to the tip region in apical cells. Thus, our results suggest common functions of PpRop and PpRopGEF in the tip-growing apical cells and the importance of a conserved Rop/RAC signaling module in the control of cell polarity in land plants.
巻・号 544(2)
ページ 241-7
公開日 2014-7-10
DOI 10.1016/j.gene.2014.04.057
PII S0378-1119(14)00488-0
PMID 24769554
MeSH Amino Acid Sequence Bryopsida / cytology* Bryopsida / genetics* Cell Polarity / genetics* Plants, Genetically Modified Sequence Alignment Signal Transduction / genetics rac GTP-Binding Proteins / genetics rac GTP-Binding Proteins / physiology*
IF 2.984
引用数 11
WOS 分野 GENETICS & HEREDITY
リソース情報
シロイヌナズナ / 植物培養細胞・遺伝子 pdp22549 pdp24435 pdp25460 pdp25667 pdr03043 pdr44434