RRC ID 49413
著者 Kanoksilapatham W, Pasomsup P, Keawram P, Cuecas A, Portillo MC, Gonzalez JM.
タイトル Fervidobacterium thailandense sp. nov., an extremely thermophilic bacterium isolated from a hot spring.
ジャーナル Int J Syst Evol Microbiol
Abstract Strain FC2004T, a strictly anaerobic, extremely thermophilic heterotroph, was isolated from a hot spring in Thailand. Typical cells of strain FC2004T were rod shaped (0.5-0.6×1.1-2.5 µm) with an outer membrane swelling out over an end. Filaments (10-30 µm long) and membrane-bound spheroids containing two or more cells inside (3-8 µm in diameter) were observed. The temperature range for growth was 60-88°C (optimum 78-80°C), pH range was 6.5-8.5 (optimum pH 7.5) and NaCl concentration range was 0 to <5 g l-1 (optimum 0.5 g l-1). S0 stimulated growth yield. S2O32- and NO3- did not influence growth. Glucose, maltose, sucrose, fructose, cellobiose, CM-cellulose and starch were utilized for growth. The membrane was composed mainly of the saturated fatty acids C16:0 and C18:0. The DNA G+C content was 45.8 mol%. The 16S rRNA gene sequence of strain FC2004T revealed highest similarity to species of the genus Fervidobacterium: F. pennivorans DSM 9078T (97-96 %), F. islandicum AW-1 (96 %), F. changbaicum CBS-1T (96 %), F. islandicum H21T (95 %), F. nodosum Rt17-B1T (95 %), F. riparium 1445tT (95 %) and F. gondwanense AB39T (93 %). Phylogenetic analysis of 16S rRNA gene sequences and average nucleotide identity analysis suggested that strain FC2004T represented a novel species within the genus Fervidobacterium, for which the name Fervidobacterium thailandense sp. nov. is proposed. The type strain is FC2004T (=JCM 18757T=ATCC BAA-2483T).
巻・号 66(12)
ページ 5023-5027
公開日 2016-12-1
DOI 10.1099/ijsem.0.001463
PMID 27566409
MeSH Bacteria / chemistry* Bacteria / genetics Bacteria / isolation & purification Bacterial Typing Techniques Base Composition DNA, Bacterial / genetics DNA, Ribosomal / genetics Fatty Acids / chemistry Geologic Sediments / microbiology Hot Springs / microbiology* Hot Temperature Phylogeny* RNA, Ribosomal, 16S / genetics Sequence Analysis, DNA Thailand
IF 2.415
引用数 7
リソース情報
一般微生物 JCM 18757