論文 - 詳細
RRC ID | 48710 |
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著者 | Guionet A, Hosseini B, Teissié J, Akiyama H, Hosseini H. |
タイトル | A new mechanism for efficient hydrocarbon electro-extraction from Botryococcus braunii. |
ジャーナル | Biotechnol Biofuels |
Abstract |
BACKGROUND:Recent understanding that specific algae have high hydrocarbon production potential has attracted considerable attention. Botryococcus braunii is a microalga with an extracellular hydrocarbon matrix, which makes it an appropriate green energy source. RESULTS:This study focuses on extracting oil from the microalgae matrix rather than the cells, eliminating the need for an excessive electric field to create electro-permeabilization. In such a way, technical limitations due to high extraction energy and cost can be overcome. Here, nanosecond pulsed electric fields (nsPEF) with 80 ns duration and 20-65 kV/cm electric fields were applied. To understand the extraction mechanism, the structure of the algae was accurately studied under fluorescence microscope; extraction was quantified using image analysis; quality of extraction was examined by thin-layer chromatography (TLC); and the cell/matrix separation was observed real-time under a microscope during nsPEF application. Furthermore, optimization was carried out by screening values of electric fields, pulse repetition frequencies, and energy spent. CONCLUSIONS:The results offer a novel method applicable for fast and continues hydrocarbon extraction process at low energy cost. |
巻・号 | 10 |
ページ | 39 |
公開日 | 2017-2-13 |
DOI | 10.1186/s13068-017-0724-1 |
PII | 724 |
PMID | 28228843 |
PMC | PMC5307828 |
IF | 4.815 |
引用数 | 11 |
WOS 分野 | BIOTECHNOLOGY & APPLIED MICROBIOLOGY ENERGY & FUELS |
リソース情報 | |
藻類 | NIES-836 |