RRC ID 48710
著者 Guionet A, Hosseini B, Teissié J, Akiyama H, Hosseini H.
タイトル A new mechanism for efficient hydrocarbon electro-extraction from Botryococcus braunii.
ジャーナル Biotechnol Biofuels
Abstract BACKGROUND:Recent understanding that specific algae have high hydrocarbon production potential has attracted considerable attention. Botryococcus braunii is a microalga with an extracellular hydrocarbon matrix, which makes it an appropriate green energy source.
RESULTS:This study focuses on extracting oil from the microalgae matrix rather than the cells, eliminating the need for an excessive electric field to create electro-permeabilization. In such a way, technical limitations due to high extraction energy and cost can be overcome. Here, nanosecond pulsed electric fields (nsPEF) with 80 ns duration and 20-65 kV/cm electric fields were applied. To understand the extraction mechanism, the structure of the algae was accurately studied under fluorescence microscope; extraction was quantified using image analysis; quality of extraction was examined by thin-layer chromatography (TLC); and the cell/matrix separation was observed real-time under a microscope during nsPEF application. Furthermore, optimization was carried out by screening values of electric fields, pulse repetition frequencies, and energy spent.
CONCLUSIONS:The results offer a novel method applicable for fast and continues hydrocarbon extraction process at low energy cost.
巻・号 10
ページ 39
公開日 2017-2-13
DOI 10.1186/s13068-017-0724-1
PII 724
PMID 28228843
PMC PMC5307828
IF 4.815
引用数 11
WOS 分野 BIOTECHNOLOGY & APPLIED MICROBIOLOGY ENERGY & FUELS
リソース情報
藻類 NIES-836