RRC ID 27710
著者 Armache JP, Anger AM, Márquez V, Franckenberg S, Fröhlich T, Villa E, Berninghausen O, Thomm M, Arnold GJ, Beckmann R, Wilson DN.
タイトル Promiscuous behaviour of archaeal ribosomal proteins: implications for eukaryotic ribosome evolution.
ジャーナル Nucleic Acids Res
Abstract In all living cells, protein synthesis occurs on ribonucleoprotein particles called ribosomes. Molecular models have been reported for complete bacterial 70S and eukaryotic 80S ribosomes; however, only molecular models of large 50S subunits have been reported for archaea. Here, we present a complete molecular model for the Pyrococcus furiosus 70S ribosome based on a 6.6 Å cryo-electron microscopy map. Moreover, we have determined cryo-electron microscopy reconstructions of the Euryarchaeota Methanococcus igneus and Thermococcus kodakaraensis 70S ribosomes and Crenarchaeota Staphylothermus marinus 50S subunit. Examination of these structures reveals a surprising promiscuous behavior of archaeal ribosomal proteins: We observe intersubunit promiscuity of S24e and L8e (L7ae), the latter binding to the head of the small subunit, analogous to S12e in eukaryotes. Moreover, L8e and L14e exhibit intrasubunit promiscuity, being present in two copies per archaeal 50S subunit, with the additional binding site of L14e analogous to the related eukaryotic r-protein L27e. Collectively, these findings suggest insights into the evolution of eukaryotic ribosomal proteins through increased copy number and binding site promiscuity.
巻・号 41(2)
ページ 1284-93
公開日 2013-1-1
DOI 10.1093/nar/gks1259
PII gks1259
PMID 23222135
PMC PMC3553981
MeSH Archaeal Proteins / chemistry* Archaeal Proteins / classification Binding Sites Cryoelectron Microscopy Desulfurococcaceae / chemistry Eukaryota / chemistry Euryarchaeota / chemistry Evolution, Molecular Models, Molecular Pyrococcus furiosus / chemistry Ribosomal Proteins / chemistry* Ribosomal Proteins / classification Ribosome Subunits, Large, Archaeal / chemistry Ribosomes / chemistry*
IF 11.502
引用数 30
WOS 分野 BIOCHEMISTRY & MOLECULAR BIOLOGY
リソース情報
一般微生物 JCM 12380