RRC ID 43762
著者 Oguri E, Miki Y, Hirano K, Yamanaka M, Beppu M.
タイトル Clearance of CD43-capped cells by macrophages: capping alone leads to phagocytosis.
ジャーナル Biol Pharm Bull
Abstract Apoptotic cells must be recognized early for phagocytosis to ensure that their toxic contents do not damage neighboring cells. In some cases this is achieved via CD43-capped membrane glycoproteins, the sialylpolylactosaminyl chains of which serve as ligands for phagocytosis by macrophages. However, because many additional changes occur during apoptosis, determining exactly which events are responsible for signaling macrophages to initiate phagocytosis remains a challenge. Here, we examined one clearance mechanism in detail and determined that capping of CD43 alone is sufficient to initiate phagocytosis. We induced macrophage-mediated phagocytosis by using cytochalasin B to artificially cap CD43 on healthy (non-apoptotic) Jurkat cells. Additional experiments confirmed that sialylpolylactosaminyl chains formed through this capping method are a prerequisite for removal, and that nucleolin is the macrophage receptor responsible for their detection. These findings strongly suggest that capping of CD43 presents a sufficient signal for phagocytosis without any additional membrane changes.
巻・号 35(4)
ページ 551-8
公開日 2012-1-1
DOI 10.1248/bpb.35.551
PII JST.JSTAGE/bpb/35.551
PMID 22466560
MeSH Apoptosis Cytochalasin B / pharmacology* HEK293 Cells Humans Jurkat Cells Leukosialin / immunology* Macrophages / immunology* Phagocytosis / immunology* Phosphoproteins / immunology RNA-Binding Proteins / immunology
IF 1.863
引用数 6
WOS 分野 PHARMACOLOGY & PHARMACY
リソース情報
ヒト・動物細胞 Jurkat(RCB0806)