RRC ID |
33523
|
著者 |
Takayama M, Koike S, Kusano M, Matsukura C, Saito K, Ariizumi T, Ezura H.
|
タイトル |
Tomato Glutamate Decarboxylase Genes SlGAD2 and SlGAD3 Play Key Roles in Regulating γ-Aminobutyric Acid Levels in Tomato (Solanum lycopersicum).
|
ジャーナル |
Plant Cell Physiol
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Abstract |
Tomato (Solanum lycopersicum) can accumulate relatively high levels of γ-aminobutyric acid (GABA) during fruit development. However, the molecular mechanism underlying GABA accumulation and its physiological function in tomato fruits remain elusive. We previously identified three tomato genes (SlGAD1, SlGAD2 and SlGAD3) encoding glutamate decarboxylase (GAD), likely the key enzyme for GABA biosynthesis in tomato fruits. In this study, we generated transgenic tomato plants in which each SlGAD was suppressed and those in which all three SlGADs were simultaneously suppressed. A significant decrease in GABA levels, i.e. 50-81% compared with wild-type (WT) levels, was observed in mature green (MG) fruits of the SlGAD2-suppressed lines, while a more drastic reduction (up to <10% of WT levels) was observed in the SlGAD3- and triple SlGAD-suppressed lines. These findings suggest that both SlGAD2 and SlGAD3 expression are crucial for GABA biosynthesis in tomato fruits. The importance of SlGAD3 expression was also confirmed by generating transgenic tomato plants that over-expressed SlGAD3. The MG and red fruits of the over-expressing transgenic lines contained higher levels of GABA (2.7- to 5.2-fold) than those of the WT. We also determined that strong down-regulation of the SlGADs had little effect on overall plant growth, fruit development or primary fruit metabolism under normal growth conditions.
|
巻・号 |
56(8)
|
ページ |
1533-45
|
公開日 |
2015-8-1
|
DOI |
10.1093/pcp/pcv075
|
PII |
pcv075
|
PMID |
26009591
|
MeSH |
Down-Regulation
Fruit / enzymology
Fruit / genetics
Fruit / physiology
Gene Expression Regulation, Plant*
Glutamate Decarboxylase / genetics*
Glutamate Decarboxylase / metabolism
Plant Proteins / genetics
Plant Proteins / metabolism
Plants, Genetically Modified
Solanum lycopersicum / enzymology*
Solanum lycopersicum / genetics
Solanum lycopersicum / physiology
gamma-Aminobutyric Acid / metabolism*
|
IF |
4.062
|
引用数 |
18
|
WOS 分野
|
PLANT SCIENCES
CELL BIOLOGY
|
リソース情報 |
トマト |
TOMJPF00001 |