RRC ID |
1536
|
著者 |
Suemori H, Yasuchika K, Hasegawa K, Fujioka T, Tsuneyoshi N, Nakatsuji N.
|
タイトル |
Efficient establishment of human embryonic stem cell lines and long-term maintenance with stable karyotype by enzymatic bulk passage.
|
ジャーナル |
Biochem Biophys Res Commun
|
Abstract |
Human ES (hES) cell lines are considered to be a valuable resource for medical research and for applications in cell therapy and drug discovery. For such utilization of hES cells to be realized, however, protocols involved in the use of hES cells, such as those for establishment, propagation, and cryopreservation, have still to be improved. Here, we report on an efficient method for the establishment of hES cell lines and its detailed characterization. Additionally, we developed a new bulk-passaging technique that preserves the karyotypic integrity of hES cell lines when maintained in culture for up to 2 years. Finally, we show that a simplified vitrification cryopreservation technique is vastly superior to standard slow-cooling methods with respect to cell viability. These results provide valuable information that will assist in achieving the goal of the large-scale hES cell culture required for the application of hES cells to disease therapy.
|
巻・号 |
345(3)
|
ページ |
926-32
|
公開日 |
2006-7-7
|
DOI |
10.1016/j.bbrc.2006.04.135
|
PII |
S0006-291X(06)00936-3
|
PMID |
16707099
|
MeSH |
Animals
Cell Culture Techniques / methods*
Cell Differentiation
Cell Line
Embryo, Mammalian / cytology*
Female
Humans
Karyotyping / methods*
Male
Mice
Mice, SCID
Stem Cells / cytology*
Teratoma / metabolism
|
IF |
2.985
|
引用数 |
250
|
WOS 分野
|
BIOPHYSICS
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
リソース情報 |
ヒトES細胞 |
KhES-1
KhES-2
KhES-3 |