RRC ID 2433
著者 Kuroda T, Tada M, Kubota H, Kimura H, Hatano SY, Suemori H, Nakatsuji N, Tada T.
タイトル Octamer and Sox elements are required for transcriptional cis regulation of Nanog gene expression.
ジャーナル Mol Cell Biol
Abstract The pluripotential cell-specific gene Nanog encodes a homeodomain-bearing transcription factor required for maintaining the undifferentiated state of stem cells. However, the molecular mechanisms that regulate Nanog gene expression are largely unknown. To address this important issue, we used luciferase assays to monitor the relative activities of deletion fragments from the 5'-flanking region of the gene. An adjacent pair of highly conserved Octamer- and Sox-binding sites was found to be essential for activating pluripotential state-specific gene expression. Furthermore, the 5'-end fragment encompassing the Octamer/Sox element was sufficient for inducing the proper expression of a green fluorescent protein reporter gene even in human embryonic stem (ES) cells. The potential of OCT4 and SOX2 to bind to this element was verified by electrophoretic mobility shift assays with extracts from F9 embryonal carcinoma cells and embryonic germ cells derived from embryonic day 12.5 embryos. However, in ES cell extracts, a complex of OCT4 with an undefined factor preferentially bound to the Octamer/Sox element. Thus, Nanog transcription may be regulated through an interaction between Oct4 and Sox2 or a novel pluripotential cell-specific Sox element-binding factor which is prominent in ES cells.
巻・号 25(6)
ページ 2475-85
公開日 2005-3-1
DOI 10.1128/MCB.25.6.2475-2485.2005
PII 25/6/2475
PMID 15743839
PMC PMC1061601
MeSH 5' Flanking Region / genetics Animals Base Sequence Binding Sites / genetics DNA-Binding Proteins / genetics* DNA-Binding Proteins / metabolism DNA-Binding Proteins / physiology* Electrophoretic Mobility Shift Assay Embryo, Mammalian / cytology Gene Expression Regulation* Genes, Reporter / genetics Green Fluorescent Proteins / analysis Green Fluorescent Proteins / genetics HMGB Proteins Homeodomain Proteins / genetics* Humans Luciferases / analysis Luciferases / genetics Mice Molecular Sequence Data Mutation / genetics Nanog Homeobox Protein Nuclear Proteins / genetics Nuclear Proteins / metabolism Nuclear Proteins / physiology* Octamer Transcription Factor-3 Response Elements / genetics* SOXB1 Transcription Factors Sequence Deletion / genetics Stem Cells / metabolism* Transcription Factors / genetics Transcription Factors / metabolism Transcription Factors / physiology* Transcription, Genetic
IF 3.611
引用数 361
WOS 分野 BIOCHEMISTRY & MOLECULAR BIOLOGY CELL BIOLOGY
リソース情報
ヒトES細胞 KhES-1