論文 - 詳細
| RRC ID | 2442 |
|---|---|
| 著者 | Senju S, Suemori H, Zembutsu H, Uemura Y, Hirata S, Fukuma D, Matsuyoshi H, Shimomura M, Haruta M, Fukushima S, Matsunaga Y, Katagiri T, Nakamura Y, Furuya M, Nakatsuji N, Nishimura Y. |
| タイトル | Genetically manipulated human embryonic stem cell-derived dendritic cells with immune regulatory function. |
| ジャーナル | Stem Cells |
| Abstract |
Genetically manipulated dendritic cells (DC) are considered to be a promising means for antigen-specific immune therapy. This study reports the generation, characterization, and genetic modification of DC derived from human embryonic stem (ES) cells. The human ES cell-derived DC (ES-DC) expressed surface molecules typically expressed by DC and had the capacities to stimulate allogeneic T lymphocytes and to process and present protein antigen in the context of histocompatibility leukocyte antigen (HLA) class II molecule. Genetic modification of human ES-DC can be accomplished without the use of viral vectors, by the introduction of expression vector plasmids into undifferentiated ES cells by electroporation and subsequent induction of differentiation of the transfectant ES cell clones to ES-DC. ES-DC introduced with invariant chain-based antigen-presenting vectors by this procedure stimulated HLA-DR-restricted antigen-specific T cells in the absence of exogenous antigen. Forced expression of programmed death-1-ligand-1 in ES-DC resulted in the reduction of the proliferative response of allogeneic T cells cocultured with the ES-DC. Generation and genetic modification of ES-DC from nonhuman primate (cynomolgus monkey) ES cells was also achieved by the currently established method. ES-DC technology is therefore considered to be a novel means for immune therapy. |
| 巻・号 | 25(11) |
| ページ | 2720-9 |
| 公開日 | 2007-11-1 |
| DOI | 10.1634/stemcells.2007-0321 |
| PII | 2007-0321 |
| PMID | 17690179 |
| MeSH | Animals Cell Differentiation / genetics* Cell Differentiation / immunology Cell Line Coculture Techniques Dendritic Cells / cytology Dendritic Cells / immunology* Dendritic Cells / metabolism Embryonic Stem Cells / cytology Embryonic Stem Cells / immunology* Embryonic Stem Cells / metabolism Genetic Vectors / immunology Genetic Vectors / metabolism Humans Macaca fascicularis Mice Transfection / methods |
| IF | 6.022 |
| 引用数 | 37 |
| WOS 分野 | BIOTECHNOLOGY & APPLIED MICROBIOLOGY HEMATOLOGY ONCOLOGY CELL & TISSUE ENGINEERING CELL BIOLOGY |
| リソース情報 | |
| ヒトES細胞 | KhES-1 KhES-3 |