RRC ID 6136
著者 Adachi K, Suemori H, Yasuda SY, Nakatsuji N, Kawase E.
タイトル Role of SOX2 in maintaining pluripotency of human embryonic stem cells.
ジャーナル Genes Cells
Abstract Human embryonic stem cell (ESC) pluripotency is thought to be regulated by several key transcription factors including OCT4, NANOG, and SOX2. Although the functions of OCT4 and NANOG in human ESCs are well defined, that of SOX2 has not been fully characterized. To investigate the role of SOX2, we modulated the level of SOX2 expression in human ESCs. Reduction of SOX2 expression in human ESCs induced trophectodermal and partial endodermal differentiation. Interestingly, CDX2, a typical trophectoderm-associated gene, was not up-regulated. In contrast, using the Tet-on gene inducible system, SOX2 over-expression in human ESCs induced trophectoderm differentiation accompanied by increased CDX2 expression. Additionally, SOX2 over-expression resulted in an increase in CGalpha-positive cells, which marks later stage trophectoderm development, rather than placental lactogen-positive cells. Thus, over-expression as well as repression of SOX2 expression in human ESCs resulted in their differentiation into the trophectoderm lineage. Our data show that SOX2 plays an important role in the maintenance of pluripotency of human ESCs and possibly, trophoblast development.
巻・号 15(5)
ページ 455-70
公開日 2010-5-1
DOI 10.1111/j.1365-2443.2010.01400.x
PII GTC1400
PMID 20384793
MeSH Animals Cell Differentiation / physiology Embryonic Stem Cells / cytology Embryonic Stem Cells / physiology* Homeodomain Proteins / genetics Homeodomain Proteins / metabolism Humans Mice Nanog Homeobox Protein Octamer Transcription Factor-3 / genetics Octamer Transcription Factor-3 / metabolism Pluripotent Stem Cells / cytology Pluripotent Stem Cells / physiology* RNA Interference RNA, Small Interfering / genetics RNA, Small Interfering / metabolism SOXB1 Transcription Factors / genetics SOXB1 Transcription Factors / metabolism* Trophoblasts / cytology Trophoblasts / metabolism
IF 1.655
引用数 91
WOS 分野 GENETICS & HEREDITY CELL BIOLOGY
リソース情報
ヒトES細胞 KhES-1