RRC ID 49507
著者 Azetsu Y, Inohaya K, Takano Y, Kinoshita M, Tasaki M, Kudo A.
タイトル The sp7 gene is required for maturation of osteoblast-lineage cells in medaka (Oryzias latipes) vertebral column development.
ジャーナル Dev Biol
Abstract Sp7 is a zinc finger transcription factor that is essential for osteoblast differentiation in mammals. To verify the characteristic features of osteoblast-lineage cells in teleosts, we established medaka sp7 mutants using a transcription activator-like effector nuclease (TALEN) genome editing system. These mutants showed severe defects in the formation of skeletal structures. In particular, the neural and the hemal arches were not formed, although the chordal centra were formed. Analysis of the transgenic medaka revealed that sp7 mutant had normal distribution of type X collagen a1 a (col10a1a)-positive osteoblast-like cells around the centrum and at the proximal region of the vertebral arch. The sp7 mutant phenotype could be rescued by exogenous sp7 expression in col10a1a-positive cells, as well as in sp7-positive osteoblast cells. Furthermore, runx2-positive osteoblast progenitors were observed on the vertebral arches, but not on the centrum, during vertebral column development. In addition, these osteoblast progenitors differentiated into the col10a1a-positive cells. In sp7 mutant, the runx2-positive cells were normally distributed at the region of unformed vertebral arch but failed to differentiate into col10a1a-positive cells. These results indicate that osteoblast-lineage cells undergo two distinct differentiation processes during development of the vertebral arch and the centrum. Nevertheless, our results verified that sp7 gene expression in osteoblast-lineage cells is required for differentiation into mature osteoblasts to form the vertebral column and other skeletal structures.
巻・号 431(2)
ページ 252-262
公開日 2017-11-15
DOI 10.1016/j.ydbio.2017.09.010
PII S0012-1606(17)30045-3
PMID 28899668
MeSH Alkaline Phosphatase / metabolism Animals Cell Differentiation / genetics* Cell Lineage / genetics* Core Binding Factor Alpha 1 Subunit / metabolism Green Fluorescent Proteins / metabolism Larva / cytology Larva / metabolism Mutation / genetics Oryzias / embryology* Oryzias / genetics* Osteoblasts / cytology* Osteoblasts / enzymology Osteoblasts / metabolism Phenotype Spine / cytology* Spine / embryology* Spine / metabolism Transcription Factors / genetics* Transcription Factors / metabolism
IF 2.896
引用数 3
リソース情報
メダカ BAC clone (ola1-019D18) Fosmid clone (GOLWF no 181_n17) sp7^TAL1 (MT1240) sp7^TAL2 (MT1241) cab runx2-EGFP (TG1397)