RRC ID 48001
著者 Lee JH, Lee CW, Park SH, Choe KM.
タイトル Spatiotemporal regulation of cell fusion by JNK and JAK/STAT signaling during Drosophila wound healing.
ジャーナル J Cell Sci
Abstract Cell-cell fusion is widely observed during development and disease, and imposes a dramatic change on participating cells. Cell fusion should be tightly controlled, but the underlying mechanism is poorly understood. Here, we found that the JAK/STAT pathway suppressed cell fusion during wound healing in the Drosophila larval epidermis, restricting cell fusion to the vicinity of the wound. In the absence of JAK/STAT signaling, a large syncytium containing a 3-fold higher number of nuclei than observed in wild-type tissue formed in wounded epidermis. The JAK/STAT ligand-encoding genes upd2 and upd3 were transcriptionally induced by wounding, and were required for suppressing excess cell fusion. JNK (also known as Basket in flies) was activated in the wound vicinity and activity peaked at ∼8 h after injury, whereas JAK/STAT signaling was activated in an adjoining concentric ring and activity peaked at a later stage. Cell fusion occurred primarily in the wound vicinity, where JAK/STAT activation was suppressed by fusion-inducing JNK signaling. JAK/STAT signaling was both necessary and sufficient for the induction of βPS integrin (also known as Myospheroid) expression, suggesting that the suppression of cell fusion was mediated at least in part by integrin protein.
巻・号 130(11)
ページ 1917-1928
公開日 2017-6-1
DOI 10.1242/jcs.187658
PII jcs.187658
PMID 28424232
MeSH Animals Cell Fusion Cell Movement Drosophila Proteins / genetics* Drosophila Proteins / metabolism Drosophila melanogaster / genetics Drosophila melanogaster / growth & development Drosophila melanogaster / metabolism* Epidermis / injuries Epidermis / metabolism* Epithelial Cells / metabolism Epithelial Cells / pathology Gene Expression Regulation Giant Cells / cytology Giant Cells / metabolism Integrin beta Chains / genetics Integrin beta Chains / metabolism Janus Kinases / genetics* Janus Kinases / metabolism Larva / genetics Larva / growth & development Larva / metabolism* MAP Kinase Kinase 4 / genetics* MAP Kinase Kinase 4 / metabolism STAT Transcription Factors / genetics* STAT Transcription Factors / metabolism Signal Transduction Transcription Factors / genetics* Transcription Factors / metabolism Wound Healing / genetics*
IF 4.573
引用数 6
WOS 分野 CELL BIOLOGY
リソース情報
ショウジョウバエ 5988R-3 5680R-1