RRC ID 48748
著者 Wang M, Jiang L, Da Q, Liu J, Feng D, Wang J, Wang HB, Jin HL.
タイトル DELAYED GREENING 238, a Nuclear-Encoded Chloroplast Nucleoid Protein, Is Involved in the Regulation of Early Chloroplast Development and Plastid Gene Expression in Arabidopsis thaliana.
ジャーナル Plant Cell Physiol
Abstract Chloroplast development is an essential process for plant growth that is regulated by numerous proteins. Plastid-encoded plastid RNA polymerase (PEP) is a large complex that regulates plastid gene transcription and chloroplast development. However, many proteins in this complex remain to be identified. Here, through large-scale screening of Arabidopsis mutants by Chl fluorescence imaging, we identified a novel protein, DELAYED GREENING 238 (DG238), which is involved in regulating chloroplast development and plastid gene expression. Loss of DG238 retards plant growth, delays young leaf greening, affects chloroplast development and lowers photosynthetic efficiency. Moreover, blue-native PAGE (BN-PAGE) and Western blot analysis indicated that PSII and PSI protein levels are reduced in dg238 mutants. DG238 is mainly expressed in young tissues and is regulated by light signals. Subcellular localization analysis showed that DG238 is a nuclear-encoded chloroplast nucleoid protein. More interestingly, DG238 was co-expressed with FLN1, which encodes an essential subunit of the PEP complex. Bimolecular fluorescence complementation (BiFC) and co-immunoprecipitation (Co-IP) assays showed that DG238 can also interact with FLN1. Taken together, these results suggest that DG238 may function as a component of the PEP complex that is important for the early stage of chloroplast development and helps regulate PEP-dependent plastid gene expression.
巻・号 57(12)
ページ 2586-2599
公開日 2016-12-1
DOI 10.1093/pcp/pcw172
PII pcw172
PMID 27818379
MeSH Arabidopsis / enzymology Arabidopsis / genetics* Arabidopsis / radiation effects Arabidopsis / ultrastructure Arabidopsis Proteins / genetics Arabidopsis Proteins / metabolism* Chloroplasts / enzymology Chloroplasts / ultrastructure* DNA-Directed RNA Polymerases / genetics DNA-Directed RNA Polymerases / metabolism* Gene Expression Regulation, Plant Light Mutation Nuclear Proteins / genetics Nuclear Proteins / metabolism* Phosphotransferases (Alcohol Group Acceptor) / genetics Phosphotransferases (Alcohol Group Acceptor) / metabolism* Phylogeny Plant Leaves / enzymology Plant Leaves / genetics Plant Leaves / radiation effects Plant Leaves / ultrastructure Plastids / enzymology Plastids / ultrastructure Protein Transport Transcription, Genetic
IF 4.062
引用数 4
WOS 分野 CELL BIOLOGY PLANT SCIENCES
リソース情報
シロイヌナズナ / 植物培養細胞・遺伝子 pss10000