RRC ID |
15320
|
著者 |
Kobayashi M, Kouzu N, Inami A, Toyooka K, Konishi Y, Matsuoka K, Matoh T.
|
タイトル |
Characterization of Arabidopsis CTP:3-deoxy-D-manno-2-octulosonate cytidylyltransferase (CMP-KDO synthetase), the enzyme that activates KDO during rhamnogalacturonan II biosynthesis.
|
ジャーナル |
Plant Cell Physiol
|
Abstract |
In plant cells, boron (B) occurs predominantly as a borate ester associated with rhamnogalacturonan II (RG-II), but the function of this B-RG-II complex has yet to be investigated. 3-Deoxy-D-manno-2-octulosonic acid (KDO) is a specific component monosaccharide of RG-II. Mutant plants defective in KDO biosynthesis are expected to have altered RG-II structure, and would be useful for studying the physiological function of the B-RG-II complex. Here, we characterized Arabidopsis CTP:KDO cytidylyltransferase (CMP-KDO synthetase; CKS), the enzyme activating KDO as a nucleotide sugar prior to its incorporation into RG-II. Our analyses localized the Arabidopsis CKS protein to mitochondria. The Arabidopsis CKS gene occurs as a single-copy gene in the genome, and we could not obtain cks null mutants from T-DNA insertion lines. Analysis using +/cks heterozygotes in the quartet1 background demonstrated that the cks mutation rendered pollen infertile through the inhibition of pollen tube elongation. These results suggest that KDO is an indispensable component of RG-II, and that the complete B-RG-II complex is essential for the cell wall integrity of rapidly growing tissues.
|
巻・号 |
52(10)
|
ページ |
1832-43
|
公開日 |
2011-10-1
|
DOI |
10.1093/pcp/pcr120
|
PII |
pcr120
|
PMID |
21893514
|
MeSH |
Amino Acid Sequence
Arabidopsis / cytology
Arabidopsis / enzymology*
Arabidopsis / ultrastructure
Chromosome Segregation / genetics
DNA, Plant / genetics
Genotype
Germ Cells, Plant / metabolism
Molecular Sequence Data
Mutagenesis, Insertional / genetics
Mutation / genetics
Nucleotidyltransferases / chemistry
Nucleotidyltransferases / metabolism*
Nucleotidyltransferases / ultrastructure
Pectins / biosynthesis*
Pollen Tube / cytology
Pollen Tube / growth & development
Pollen Tube / metabolism
Protein Transport
Recombinant Proteins / metabolism
Sequence Alignment
Subcellular Fractions / enzymology
Sugar Acids / metabolism*
|
IF |
4.062
|
引用数 |
20
|
WOS 分野
|
PLANT SCIENCES
CELL BIOLOGY
|
リソース情報 |
シロイヌナズナ / 植物培養細胞・遺伝子 |
rpc00008 |