RRC ID 36884
著者 Kawabata K, Kato Y, Sakano T, Baba N, Hagiwara K, Tamura A, Baba S, Natsume M, Ohigashi H.
タイトル Effects of phytochemicals on in vitro anti-inflammatory activity of Bifidobacterium adolescentis.
ジャーナル Biosci Biotechnol Biochem
Abstract Probiotics have been shown to improve the condition of not only the human gastrointestinal tract but also the entire body. We found that quercetin enhances the anti-inflammatory activity of Bifidobacterium adolescentis, which is abundant in human intestines. Here, we assessed whether certain phytochemicals could enhance the anti-inflammatory activity of B. adolescentis. Bifidobacteria were anaerobically cultured with phytochemicals for 3 h, and the anti-inflammatory activity of the supernatants was estimated by testing their ability to inhibit nitric oxide (NO) production by lipopolysaccharide-stimulated RAW264 macrophages. Of the 55 phytochemicals tested, phloretin, (+)-taxifolin, and (-)-epigallocatechin gallate as well as quercetin-3-O-glucoside and quercetin-4'-O-glucoside were similar to quercetin in promoting NO suppression by B. adolescentis. In addition, the phytochemicals excluding quercetin increased the concentrations of lactic and acetic acids in the co-culture supernatants. These results suggest that some phytochemicals may activate the anti-inflammatory function of B. adolescentis.
巻・号 79(5)
ページ 799-807
公開日 2015-1-1
DOI 10.1080/09168451.2015.1006566
PMID 25721815
MeSH Acetic Acid / metabolism Animals Anti-Inflammatory Agents / pharmacology* Bifidobacterium* / drug effects Bifidobacterium* / physiology Catechin / analogs & derivatives Catechin / pharmacology Cell Line / drug effects Coculture Techniques Dose-Response Relationship, Drug Flavonoids Glucosides Lactic Acid / metabolism Lipopolysaccharides / pharmacology Macrophages / drug effects Mice Nitric Oxide / metabolism Phytochemicals / pharmacology Probiotics / pharmacology* Quercetin / analogs & derivatives Quercetin / pharmacology
IF 1.516
引用数 7
WOS 分野 FOOD SCIENCE & TECHNOLOGY BIOTECHNOLOGY & APPLIED MICROBIOLOGY CHEMISTRY, APPLIED BIOCHEMISTRY & MOLECULAR BIOLOGY
リソース情報
ヒト・動物細胞 RAW 264(RCB0535)