RRC ID 36993
著者 Yokoo T, Matsumoto K, Ooba T, Morimoto K, Taguchi S.
タイトル Enhanced poly(3-hydroxybutyrate) production in transgenic tobacco BY-2 cells using engineered acetoacetyl-CoA reductase.
ジャーナル Biosci Biotechnol Biochem
Abstract Highly active mutant of NADPH-dependent acetoacetyl-CoA reductase (PhaB) was expressed in Nicotiana tabacum cv. Bright Yellow-2 cultured cells to produce poly(3-hydroxybutyrate) [P(3HB)]. The mutated PhaB increased P(3HB) content by three-fold over the control, indicating that the mutant was a versatile tool for P(3HB) production. Additionally, the PhaB-catalyzed reaction was suggested to be a rate-limiting step of P(3HB) biosynthesis in tobacco BY-2 cells.
巻・号 79(6)
ページ 986-8
公開日 2015-1-1
DOI 10.1080/09168451.2014.1002448
PMID 25647430
MeSH Alcohol Oxidoreductases / genetics* Alcohol Oxidoreductases / metabolism Biocatalysis Cell Line Hydroxybutyrates / metabolism* Kinetics Plants, Genetically Modified Polyesters / metabolism* Protein Engineering* Tobacco / cytology* Tobacco / genetics* Transformation, Genetic
IF 1.516
引用数 3
WOS 分野 FOOD SCIENCE & TECHNOLOGY BIOTECHNOLOGY & APPLIED MICROBIOLOGY BIOCHEMISTRY & MOLECULAR BIOLOGY CHEMISTRY, APPLIED
リソース情報
シロイヌナズナ / 植物培養細胞・遺伝子 rpc00001