RRC ID 50304
著者 Wakabayashi T, Naito H, Suehiro JI, Lin Y, Kawaji H, Iba T, Kouno T, Ishikawa-Kato S, Furuno M, Takara K, Muramatsu F, Weizhen J, Kidoya H, Ishihara K, Hayashizaki Y, Nishida K, Yoder MC, Takakura N.
タイトル CD157 Marks Tissue-Resident Endothelial Stem Cells with Homeostatic and Regenerative Properties.
ジャーナル Cell Stem Cell
Abstract The generation of new blood vessels via angiogenesis is critical for meeting tissue oxygen demands. A role for adult stem cells in this process remains unclear. Here, we identified CD157 (bst1, bone marrow stromal antigen 1) as a marker of tissue-resident vascular endothelial stem cells (VESCs) in large arteries and veins of numerous mouse organs. Single CD157+ VESCs form colonies in vitro and generate donor-derived portal vein, sinusoids, and central vein endothelial cells upon transplantation in the liver. In response to injury, VESCs expand and regenerate entire vasculature structures, supporting the existence of an endothelial hierarchy within blood vessels. Genetic lineage tracing revealed that VESCs maintain large vessels and sinusoids in the normal liver for more than a year, and transplantation of VESCs rescued bleeding phenotypes in a mouse model of hemophilia. Our findings show that tissue-resident VESCs display self-renewal capacity and that vascular regeneration potential exists in peripheral blood vessels.
巻・号 22(3)
ページ 384-397.e6
公開日 2018-3-1
DOI 10.1016/j.stem.2018.01.010
PII S1934-5909(18)30010-9
PMID 29429943
MeSH ADP-ribosyl Cyclase / metabolism* Animals Antigens, CD / metabolism* Biomarkers / metabolism Blood Vessels / metabolism Cell Lineage Colony-Forming Units Assay Endothelial Progenitor Cells / cytology Endothelial Progenitor Cells / metabolism* Endothelial Progenitor Cells / transplantation Endothelial Progenitor Cells / ultrastructure Factor VIII / metabolism GPI-Linked Proteins / metabolism Homeostasis* Liver / cytology Liver / physiology Mice, Inbred C57BL Regeneration*
IF 20.86
引用数 41
リソース情報
実験動物マウス RBRC02401
ヒト・動物細胞 OP9(RCB1124)