RRC ID 30775
著者 Bueso E, Muñoz-Bertomeu J, Campos F, Brunaud V, Martínez L, Sayas E, Ballester P, Yenush L, Serrano R.
タイトル ARABIDOPSIS THALIANA HOMEOBOX25 uncovers a role for Gibberellins in seed longevity.
ジャーナル Plant Physiol
Abstract Seed longevity is crucial for agriculture and plant genetic diversity, but it is limited by cellular damage during storage. Seeds are protected against aging by cellular defenses and by structures such as the seed coat. We have screened an activation-tagging mutant collection of Arabidopsis (Arabidopsis thaliana) and selected four dominant mutants with improved seed longevity (isl1-1D to isl4-1D) under both natural and accelerated aging conditions. In the isl1-1D mutant, characterized in this work, overexpression of the transcription factor ARABIDOPSIS THALIANA HOMEOBOX25 (ATHB25; At5g65410) increases the expression of GIBBERELLIC ACID3-OXIDASE2, encoding a gibberellin (GA) biosynthetic enzyme, and the levels of GA1 and GA4 are higher (3.2- and 1.4-fold, respectively) in the mutant than in the wild type. The morphological and seed longevity phenotypes of the athb25-1D mutant were recapitulated in transgenic plants with moderate (4- to 6-fold) overexpression of ATHB25. Simultaneous knockdown of ATHB25, ATHB22, and ATHB31 expression decreases seed longevity, as does loss of ATHB25 and ATHB22 function in a double mutant line. Seeds from wild-type plants treated with GA and from a quintuple DELLA mutant (with constitutive GA signaling) are more tolerant to aging, providing additional evidence for a role of GA in seed longevity. A correlation was observed in several genotypes between seed longevity and mucilage formation at the seed surface, suggesting that GA may act by reinforcing the seed coat. This mechanism was supported by the observation of a maternal effect in reciprocal crosses between the wild type and the athb25-1D mutant.
巻・号 164(2)
ページ 999-1010
公開日 2014-2-1
DOI 10.1104/pp.113.232223
PII pp.113.232223
PMID 24335333
PMC PMC3912122
MeSH Arabidopsis / genetics Arabidopsis / growth & development Arabidopsis / metabolism* Arabidopsis Proteins / metabolism* Gene Expression Profiling Gene Expression Regulation, Plant / drug effects Genes, Plant / genetics Genotype Germination / drug effects Gibberellins / pharmacology* Mutation / genetics Nucleotide Motifs / genetics Oligonucleotide Array Sequence Analysis Phenotype Plant Mucilage / metabolism Plants, Genetically Modified Promoter Regions, Genetic / genetics Seeds / drug effects Seeds / growth & development* Seeds / metabolism* Staining and Labeling Triazoles / pharmacology
IF 6.902
引用数 39
WOS 分野 PLANT SCIENCES
リソース情報
シロイヌナズナ / 植物培養細胞・遺伝子 pda12325