RRC ID 30782
著者 Maselli GA, Slamovits CH, Bianchi JI, Vilarrasa-Blasi J, Caño-Delgado AI, Mora-García S.
タイトル Revisiting the evolutionary history and roles of protein phosphatases with Kelch-like domains in plants.
ジャーナル Plant Physiol
Abstract Protein phosphatases with Kelch-like domains (PPKL) are members of the phosphoprotein phosphatases family present only in plants and alveolates. PPKL have been described as positive effectors of brassinosteroid (BR) signaling in plants. Most of the evidence supporting this role has been gathered using one of the four homologs in Arabidopsis (Arabidopsis thaliana), brassinosteroid-insensitive1 suppressor (BSU1). We reappraised the roles of the other three members of the family, BSL1, BSL2, and BSL3, through phylogenetic, functional, and genetic analyses. We show that BSL1 and BSL2/BSL3 belong to two ancient evolutionary clades that have been highly conserved in land plants. In contrast, BSU1-type genes are exclusively found in the Brassicaceae and display a remarkable sequence divergence, even among closely related species. Simultaneous loss of function of the close paralogs BSL2 and BSL3 brings about a peculiar array of phenotypic alterations, but with marginal effects on BR signaling; loss of function of BSL1 is, in turn, phenotypically silent. Still, the products of these three genes account for the bulk of PPKL-related activity in Arabidopsis and together have an essential role in the early stages of development that BSU1 is unable to supplement. Our results underline the functional relevance of BSL phosphatases in plants and suggest that BSL2/BSL3 and BSU1 may have contrasting effects on BR signaling. Given that BSU1-type genes have likely undergone a functional shift and are phylogenetically restricted, we caution that inferences based on these genes to the whole family or to other species may be misleading.
巻・号 164(3)
ページ 1527-41
公開日 2014-3-1
DOI 10.1104/pp.113.233627
PII pp.113.233627
PMID 24492333
PMC PMC3938638
MeSH Arabidopsis / drug effects Arabidopsis / enzymology Arabidopsis / genetics Brassinosteroids / pharmacology Evolution, Molecular* Flowers / anatomy & histology Flowers / drug effects Gene Expression Regulation, Plant / drug effects Genes, Plant / genetics Mutation / genetics Phenotype Phosphoprotein Phosphatases / chemistry* Phosphoprotein Phosphatases / genetics* Phylogeny Plant Proteins / metabolism Plants / drug effects Plants / enzymology* Plants / genetics* Protein Structure, Tertiary Signal Transduction / drug effects Signal Transduction / genetics Subcellular Fractions / drug effects Subcellular Fractions / enzymology
IF 6.902
引用数 22
WOS 分野 PLANT SCIENCES
リソース情報
シロイヌナズナ / 植物培養細胞・遺伝子 pda09652 pda19723 pdz88595