RRC ID 38866
著者 van Roermund CW, Schroers MG, Wiese J, Facchinelli F, Kurz S, Wilkinson S, Charton L, Wanders RJ, Waterham HR, Weber AP, Link N.
タイトル The Peroxisomal NAD Carrier from Arabidopsis Imports NAD in Exchange with AMP.
ジャーナル Plant Physiol
Abstract Cofactors such as NAD, AMP, and Coenzyme A (CoA) are essential for a diverse set of reactions and pathways in the cell. Specific carrier proteins are required to distribute these cofactors to different cell compartments, including peroxisomes. We previously identified a peroxisomal transport protein in Arabidopsis (Arabidopsis thaliana) called the peroxisomal NAD carrier (PXN). When assayed in vitro, this carrier exhibits versatile transport functions, e.g. catalyzing the import of NAD or CoA, the exchange of NAD/NADH, and the export of CoA. These observations raise the question about the physiological function of PXN in plants. Here, we used Saccharomyces cerevisiae to address this question. First, we confirmed that PXN, when expressed in yeast, is active and targeted to yeast peroxisomes. Secondl, detailed uptake analyses revealed that the CoA transport function of PXN can be excluded under physiological conditions due to its low affinity for this substrate. Third, we expressed PXN in diverse mutant yeast strains and investigated the suppression of the mutant phenotypes. These studies provided strong evidences that PXN was not able to function as a CoA transporter or a redox shuttle by mediating a NAD/NADH exchange, but instead catalyzed the import of NAD into peroxisomes against AMP in intact yeast cells.
巻・号 171(3)
ページ 2127-39
公開日 2016-7-1
DOI 10.1104/pp.16.00540
PII pp.16.00540
PMID 27208243
PMC PMC4936582
MeSH Adenosine Monophosphate / metabolism* Arabidopsis / metabolism* Arabidopsis Proteins / genetics Arabidopsis Proteins / metabolism* Coenzyme A / metabolism Malate Dehydrogenase / genetics Malate Dehydrogenase / metabolism Mitochondrial Membrane Transport Proteins / genetics Mitochondrial Membrane Transport Proteins / metabolism* Mitochondrial Proteins NAD / metabolism* Nucleotide Transport Proteins Organic Cation Transport Proteins / genetics Peroxisomes / metabolism Recombinant Proteins / genetics Recombinant Proteins / metabolism Saccharomyces cerevisiae / genetics Saccharomyces cerevisiae Proteins / genetics Sequence Deletion
IF 6.902
引用数 22
WOS 分野 PLANT SCIENCES
リソース情報
シロイヌナズナ / 植物培養細胞・遺伝子 pda00682