RRC ID 47272
著者 Shimada N, Inami S, Sato S, Kitamoto T, Sakai T.
タイトル Modulation of light-driven arousal by LIM-homeodomain transcription factor Apterous in large PDF-positive lateral neurons of the Drosophila brain.
ジャーナル Sci Rep
Abstract Apterous (Ap), the best studied LIM-homeodomain transcription factor in Drosophila, cooperates with the cofactor Chip (Chi) to regulate transcription of specific target genes. Although Ap regulates various developmental processes, its function in the adult brain remains unclear. Here, we report that Ap and Chi in the neurons expressing PDF, a neuropeptide, play important roles in proper sleep/wake regulation in adult flies. PDF-expressing neurons consist of two neuronal clusters: small ventral-lateral neurons (s-LNvs) acting as the circadian pacemaker and large ventral-lateral neurons (l-LNvs) regulating light-driven arousal. We identified that Ap localizes to the nuclei of s-LNvs and l-LNvs. In light-dark (LD) cycles, RNAi knockdown or the targeted expression of dominant-negative forms of Ap or Chi in PDF-expressing neurons or l-LNvs promoted arousal. In contrast, in constant darkness, knockdown of Ap in PDF-expressing neurons did not promote arousal, indicating that a reduced Ap function in PDF-expressing neurons promotes light-driven arousal. Furthermore, Ap expression in l-LNvs showed daily rhythms (peaking at midnight), which are generated by a direct light-dependent mechanism rather than by the endogenous clock. These results raise the possibility that the daily oscillation of Ap expression in l-LNvs may contribute to the buffering of light-driven arousal in wild-type flies.
巻・号 6
ページ 37255
公開日 2016-11-17
DOI 10.1038/srep37255
PII srep37255
PMID 27853240
PMC PMC5112534
MeSH Animals Arousal / physiology* Brain / cytology Brain / metabolism* Cell Nucleus / genetics Cell Nucleus / metabolism* Circadian Rhythm / physiology* Drosophila Proteins / biosynthesis* Drosophila Proteins / genetics Drosophila melanogaster LIM-Homeodomain Proteins / biosynthesis* LIM-Homeodomain Proteins / genetics Light* Neurons / cytology Neurons / metabolism* Neuropeptides / biosynthesis* Neuropeptides / genetics Transcription Factors / biosynthesis* Transcription Factors / genetics
IF 3.998
引用数 4
WOS 分野 NEUROSCIENCES
リソース情報
ショウジョウバエ 8376R-1 GFP-IR-2