RRC ID 48782
著者 Miura T, Moriya H, Iwai S.
タイトル Assessing phagotrophy in the mixotrophic ciliate Paramecium bursaria using GFP-expressing yeast cells.
ジャーナル FEMS Microbiol Lett
Abstract We used cells of the yeast Saccharomyces cerevisiae expressing green fluorescent protein (GFP) as fluorescently labelled prey to assess the phagocytic activities of the mixotrophic ciliate Paramecium bursaria, which harbours symbiotic Chlorella-like algae. Because of different fluorescence spectra of GFP and algal chlorophyll, ingested GFP-expressing yeast cells can be distinguished from endosymbiotic algal cells and directly counted in individual P. bursaria cells using fluorescence microscopy. By using GFP-expressing yeast cells, we found that P. bursaria altered ingestion activities under different physiological conditions, such as different growth phases or the presence/absence of endosymbionts. Use of GFP-expressing yeast cells allowed us to estimate the digestion rates of live prey of the ciliate. In contrast to the ingestion activities, the digestion rate within food vacuoles was not affected by the presence of endosymbionts, consistent with previous findings that food and perialgal vacuoles are spatially and functionally separated in P. bursaria. Thus, GFP-expressing yeast may provide a valuable tool to assess both ingestion and digestion activities of ciliates that feed on eukaryotic organisms.
巻・号 364(12)
公開日 2017-6-7
DOI 10.1093/femsle/fnx117
PII 3861962
PMID 28591835
MeSH Chlorella vulgaris / physiology Green Fluorescent Proteins / genetics* Paramecium / genetics* Paramecium / growth & development Paramecium / physiology* Phagocytosis* Photosynthesis Saccharomyces cerevisiae / genetics* Symbiosis Vacuoles
IF 1.994
引用数 2
リソース情報
ゾウリムシ HA1g (PB034004A)