RRC ID 18948
著者 Wang Y, Hou Y, Gu H, Kang D, Chen Z, Liu J, Qu LJ.
タイトル The Arabidopsis APC4 subunit of the anaphase-promoting complex/cyclosome (APC/C) is critical for both female gametogenesis and embryogenesis.
ジャーナル Plant J
Abstract The anaphase-promoting complex/cyclosome (APC/C) is an E3 ubiquitin ligase that is involved in regulating cell-cycle progression. It has been widely studied in yeast and animal cells, but the function and regulation of the APC/C in plant cells are largely unknown. The Arabidopsis APC/C comprises at least 11 subunits, only a few of which have been studied in detail. APC4 is proposed to be a connector in the APC/C in yeast and animals. Here, we report the functional characterization of the Arabidopsis APC4 protein. We examined three heterozygous plant lines carrying apc4 alleles. These plants showed pleiotropic developmental defects in reproductive processes, including abnormal nuclear behavior in the developing embryo sac and aberrant cell division in embryos; these phenotypes differ from those reported for mutants of other subunits. Some ovules and embryos of apc4/+ plants also accumulated cyclin B protein, a known substrate of APC/C, suggesting a compromised function of APC/C. Arabidopsis APC4 was expressed in meristematic cells of seedlings, ovules in pistils and embryos in siliques, and was mainly localized in the nucleus. Additionally, the distribution of auxin was distorted in some embryos of apc4/+ plants. Our results indicate that Arabidopsis APC4 plays critical roles in female gametogenesis and embryogenesis, possibly as a connector in APC/C, and that regulation of auxin distribution may be involved in these processes.
巻・号 69(2)
ページ 227-40
公開日 2012-1-1
DOI 10.1111/j.1365-313X.2011.04785.x
PMID 21910774
MeSH Anaphase Anaphase-Promoting Complex-Cyclosome / genetics Anaphase-Promoting Complex-Cyclosome / metabolism* Arabidopsis / embryology* Arabidopsis / genetics Arabidopsis / growth & development Arabidopsis / ultrastructure Arabidopsis Proteins / genetics Arabidopsis Proteins / metabolism* Base Sequence Cell Cycle Cell Cycle Proteins Cyclin B / genetics Cyclin B / metabolism Gametogenesis, Plant / physiology* Gene Expression Regulation, Plant / genetics Genetic Complementation Test Indoleacetic Acids / metabolism Molecular Sequence Data Mutagenesis, Insertional Nuclear Proteins / genetics Nuclear Proteins / metabolism Ovule / embryology Ovule / genetics Ovule / growth & development Ovule / ultrastructure Phenotype Phylogeny Plants, Genetically Modified Schizosaccharomyces / genetics Schizosaccharomyces / metabolism Seedlings / genetics Seedlings / growth & development Seedlings / ultrastructure Seeds / genetics Seeds / growth & development Seeds / ultrastructure Sequence Analysis, DNA
IF 6.141
引用数 23
WOS 分野 PLANT SCIENCES
リソース情報
酵母 cut20‐100 , pREP41