| RRC ID |
50499
|
| 著者 |
Yamanaka H, Komeya M, Nakamura H, Sanjo H, Sato T, Yao M, Kimura H, Fujii T, Ogawa T.
|
| タイトル |
A monolayer microfluidic device supporting mouse spermatogenesis with improved visibility.
|
| ジャーナル |
Biochem Biophys Res Commun
|
| Abstract |
In our previous study, we produced a microfluidic device (MFD) which successfully maintained spermatogenesis for over 6 months in mouse testis tissues loaded in the device. In the present study, we developed a new MFD, a monolayer device (ML-D) with a barrier structure consisting of pillars and slits, which is simpler in design and easier to make. This ML-D was also effective for inducing mouse spermatogenesis and maintained it for a longer period than the conventional culture method. In addition, we devised a way of introducing sample tissue into the device during its production, just before bonding the upper layer of polydimethylsiloxane (PDMS) and bottom glass slide. The tissue can obtain nutrients horizontally from the medium running beside it and oxygen vertically from above through PDMS. In addition, the glass slide set at the bottom improved the visibility of the sample tissue with an inverted microscope. When we took photos of cultured tissue of the Acr-Gfp transgenic mouse testis in ML-D sequentially every day, morphological changes of the acrosome during spermiogenesis were successfully recorded. The ML-D is simple in design and useful for culturing testis tissue for inducing and maintaining spermatogenesis with clearer visibility. Due to the new method of sample loading, tissues other than testis should also be applicable.
|
| 巻・号 |
500(4)
|
| ページ |
885-891
|
| 公開日 |
2018-6-12
|
| DOI |
10.1016/j.bbrc.2018.04.180
|
| PII |
S0006-291X(18)30973-2
|
| PMID |
29705697
|
| MeSH |
Animals
Dimethylpolysiloxanes / chemistry
Equipment Design / instrumentation*
Gene Expression
Genes, Reporter
Green Fluorescent Proteins / genetics
Green Fluorescent Proteins / metabolism
Lab-On-A-Chip Devices*
Male
Mice
Mice, Transgenic
Spermatogenesis / genetics*
Spermatozoa / growth & development
Spermatozoa / metabolism
Spermatozoa / ultrastructure*
Testis / cytology*
Testis / growth & development
Testis / metabolism
Tissue Culture Techniques
|
| IF |
2.985
|
| 引用数 |
9
|
| リソース情報 |
| 実験動物マウス |
RBRC00445
RBRC00886 |
