RRC ID 50645
著者 Shiota M, Naya M, Yamamoto T, Hishiki T, Tani T, Takahashi H, Kubo A, Koike D, Itoh M, Ohmura M, Kabe Y, Sugiura Y, Hiraoka N, Morikawa T, Takubo K, Suina K, Nagashima H, Sampetrean O, Nagano O, Saya H, Yamazoe S, Watanabe H, Suematsu M.
タイトル Gold-nanofève surface-enhanced Raman spectroscopy visualizes hypotaurine as a robust anti-oxidant consumed in cancer survival.
ジャーナル Nat Commun
Abstract Gold deposition with diagonal angle towards boehmite-based nanostructure creates random arrays of horse-bean-shaped nanostructures named gold-nanofève (GNF). GNF generates many electromagnetic hotspots as surface-enhanced Raman spectroscopy (SERS) excitation sources, and enables large-area visualization of molecular vibration fingerprints of metabolites in human cancer xenografts in livers of immunodeficient mice with sufficient sensitivity and uniformity. Differential screening of GNF-SERS signals in tumours and those in parenchyma demarcated tumour boundaries in liver tissues. Furthermore, GNF-SERS combined with quantum chemical calculation identified cysteine-derived glutathione and hypotaurine (HT) as tumour-dominant and parenchyma-dominant metabolites, respectively. CD44 knockdown in cancer diminished glutathione, but not HT in tumours. Mechanisms whereby tumours sustained HT under CD44-knockdown conditions include upregulation of PHGDH, PSAT1 and PSPH that drove glycolysis-dependent activation of serine/glycine-cleavage systems to provide one-methyl group for HT synthesis. HT was rapidly converted into taurine in cancer cells, suggesting that HT is a robust anti-oxidant for their survival under glutathione-suppressed conditions.
巻・号 9(1)
ページ 1561
公開日 2018-4-19
DOI 10.1038/s41467-018-03899-1
PII 10.1038/s41467-018-03899-1
PMID 29674746
PMC PMC5908798
MeSH Animals Antioxidants / chemistry* Antioxidants / metabolism* Female Glutathione / metabolism Gold / chemistry* Gold / metabolism Humans Liver / chemistry Liver / metabolism Male Metal Nanoparticles / chemistry* Mice Mice, Inbred C57BL Mice, SCID Neoplasms / genetics Neoplasms / metabolism* Spectrum Analysis, Raman / instrumentation Spectrum Analysis, Raman / methods* Taurine / analogs & derivatives* Taurine / chemistry Taurine / metabolism Transaminases / genetics Transaminases / metabolism
IF 12.121
引用数 26
リソース情報
ヒト・動物細胞 MCF7(RCB1904) HCT116(RCB2979)