RRC ID 36242
著者 Kuo HK, McMahan S, Rota CM, Kohl KP, Sekelsky J.
タイトル Drosophila FANCM helicase prevents spontaneous mitotic crossovers generated by the MUS81 and SLX1 nucleases.
ジャーナル Genetics
Abstract Several helicases function during repair of double-strand breaks and handling of blocked or stalled replication forks to promote pathways that prevent formation of crossovers. Among these are the Bloom syndrome helicase BLM and the Fanconi anemia group M (FANCM) helicase. To better understand functions of these helicases, we compared phenotypes of Drosophila melanogaster Blm and Fancm mutants. As previously reported for BLM, FANCM has roles in responding to several types of DNA damage in preventing mitotic and meiotic crossovers and in promoting the synthesis-dependent strand annealing pathway for repair of a double-strand gap. In most assays, the phenotype of Fancm mutants is less severe than that of Blm mutants, and the phenotype of Blm Fancm double mutants is more severe than either single mutant, indicating both overlapping and unique functions. It is thought that mitotic crossovers arise when structure-selective nucleases cleave DNA intermediates that would normally be unwound or disassembled by these helicases. When BLM is absent, three nucleases believed to function as Holliday junction resolvases--MUS81-MMS4, MUS312-SLX1, and GEN--become essential. In contrast, no single resolvase is essential in mutants lacking FANCM, although simultaneous loss of GEN and either of the others is lethal in Fancm mutants. Since Fancm mutants can tolerate loss of a single resolvase, we were able to show that spontaneous mitotic crossovers that occur when FANCM is missing are dependent on MUS312 and either MUS81 or SLX1.
巻・号 198(3)
ページ 935-45
公開日 2014-11-1
DOI 10.1534/genetics.114.168096
PII genetics.114.168096
PMID 25205745
PMC PMC4224181
MeSH Animals Crossing Over, Genetic* DNA Damage DNA Helicases / metabolism* DNA-Binding Proteins / metabolism* Drosophila Proteins / metabolism* Drosophila melanogaster / cytology* Drosophila melanogaster / enzymology Drosophila melanogaster / genetics* Endodeoxyribonucleases / metabolism* Endonucleases / metabolism* Genome, Insect Meiosis / genetics Mitosis / genetics* Mutation / genetics Phenotype Recombinases / metabolism
IF 4.015
引用数 13
WOS 分野 GENETICS & HEREDITY
リソース情報
ショウジョウバエ DGRC#140133